Team:EPF Lausanne

Our project:
Our team wants to help stopping the propagation of malaria, a disease which has a death toll of over a million per year.
Malaria is transmitted by the parasite Plasmodium whose life cycle is well-studied: The mosquitoes are the vectors which propagate the infection to humans. The infected human will then again infect mosquitoes consuming a blood meal which then completes the cycle. Bacteria of the genus Asaia have been proven to be stably associated to a malaria propagating mosquitoe, Anopheles stephensi [1]. We plan to take advantage of this and use engineered Asaia to block the Plasmodium cycle.

Our progress:
We are now in our first week in the wet lab. Apart from training basic lab skills like making a PCR and running a gel we are growing Asaia and studying its properties. Furthermore we finished our first biobrick containing an origin of replication for Asaia. Within the next week we also plan to test the propagation of Asaia within Drosophila.

More on Asaia and on our plan:
The bacterium Asaia was chosen as a chassis mainly since it is naturally present in the mosquitoes gut. Furthermore it is transmitted vertically (to the offspring) and horizontally (during reproduction) which enables the engineered Asaia to propagate within a mosquito population. We have discussed several ways of blocking the Plasmodium in the gut of the mosquito. We plan to test the following two approaches:

• Asaia produces an immunotoxin consisting of an antibody, which will bind to a specific site on the plasmodium and a toxin (a porin, which will perforate the cellular membrane of the parasite).

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