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Team:Tsinghua/Notebook/17 September 2010
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(Difference between revisions)
(New page: == Module Ⅱ, group 4 == According to the known DNA sequence of OmpA and CBD, design the primer for the following Two-step PCR. primer design： OmpA Upstream primer: TCATCATATGAAA...) |
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Downstream primer: ACGCCAGTACCTGCAAGCTTTGAGCTCCCTCCTCCAGA | Downstream primer: ACGCCAGTACCTGCAAGCTTTGAGCTCCCTCCTCCAGA | ||
| - | + | CBD | |
| - | Upstream primer: ggagggagctcaAAGCTTGCAGGTACTGGCGTCGTATC | + | Upstream primer: ggagggagctcaAAGCTTGCAGGTACTGGCGTCGTATC |
| - | Downstream primer: ACTGCTCGAGAGTTGAACCGTAAGCAAGAGTTCC | + | Downstream primer: ACTGCTCGAGAGTTGAACCGTAAGCAAGAGTTCC |
NOTE：the downstream primer of OmpA and the upstream primer of CBD will be matching during the PCR, so the Two-step PCR can be realized. | NOTE：the downstream primer of OmpA and the upstream primer of CBD will be matching during the PCR, so the Two-step PCR can be realized. | ||
Latest revision as of 02:56, 28 October 2010
Module Ⅱ, group 4
According to the known DNA sequence of OmpA and CBD, design the primer for the following Two-step PCR.
primer design：
OmpA
Upstream primer: TCATCATATGAAAGCTACTAAACTGGTACTGG Downstream primer: ACGCCAGTACCTGCAAGCTTTGAGCTCCCTCCTCCAGA
CBD
Upstream primer: ggagggagctcaAAGCTTGCAGGTACTGGCGTCGTATC Downstream primer: ACTGCTCGAGAGTTGAACCGTAAGCAAGAGTTCC
NOTE：the downstream primer of OmpA and the upstream primer of CBD will be matching during the PCR, so the Two-step PCR can be realized.
Order the designed preimers.After getting the primer,Use the K-up and K-do to detect the P+K+C. Meanwhile use the digestion protocol to ensure the result.
