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Team:Valencia/Notebook/July
From 2010.igem.org
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We filled up the petri dished with the yeast liquid culture medium. | We filled up the petri dished with the yeast liquid culture medium. | ||
Then we planted the yeasts into the petri dishes. | Then we planted the yeasts into the petri dishes. | ||
| - | We prepared a overnight culture at 37ºC into a shaker with the bacteria having the plasmids pG1 and pLZ in order to purificate them next | + | We prepared a overnight culture at 37ºC into a shaker with the bacteria having the plasmids pG1 and pLZ in order to purificate them next Monday. |
=July 11th= | =July 11th= | ||
Revision as of 10:44, 13 July 2010
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Contents |
July 8th
WETLab meeting
We start to put toghether all the thing, to begin working in the Lab!! Also made plans for the future.
We prepared the cultive medium for growing our Yeasts.
Social Event
We got together in Serranos Towers, to chill out a litle bit after the "hard work" in the lab.
July 9th
We filled up the petri dished with the yeast liquid culture medium. Then we planted the yeasts into the petri dishes. We prepared a overnight culture at 37ºC into a shaker with the bacteria having the plasmids pG1 and pLZ in order to purificate them next Monday.
July 11th
Spain won the World cup!!!!
July 12th
We performed a Miniprep, to purify the plasmids pG1 and pLZ, then we sarted a digest for verify we have the plasmids and its concentration. We stored the rest of the plasmids in the refrigerator (maybe we want to use it later!!!) We made a liquid culture of the E.Coli strains with the LEA gene, and puted it into the stove at 37ºC.
