Team:Newcastle/Meetings/12 July 2010

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Revision as of 08:53, 13 July 2010

Chair: Da, Minutes: Younus, Comp

Cloning Strategies

There are 8 cloning strategies which are required:

SR1 - finished!
YneA - biobrick nearly coming back
LacI - not sure how to test this biobrick
2 for Swarming (sfp and swrA) - cloning strategies nearly finished
2 for Subtilin (immunity and production) - cloning strategies nearly finished
RocF (need to use Gibson method) - not started

So only the cloning strategy for SR1 is finished. 7 more to go!

YneA - our most important BB - Phil, Rach, Deena, Younus
Swarming (2) - Harsh, Alan, Steve, Da
LacI - Phil, Younus, Rachel
Subtilin (2) - Harsh, Alan, Steve, Deena
RocF - Harsh, Alan, Steve, Da

5 parts to a Cloning Strategy:

Purpose and justification
Modelling
Construction
Cloning and Integration
Testing and Characterising

Wiki

Labbook

. Aim
. Materials and Methods
. Procedure/Protocol
. Results
. Conclusion

Everyone needs to carry out this protocol above Photographs add and label them In charge of wiki - Deena and Rachel (everyone everyday they have to send the links to both of them to check)

20th July - UK iGEM Gathering at Newcastle!

Presentation

Introduction - Rach, Deena, Jannetta

Cover Page
Picture of standing us up
Introduction of our iGEM project - using BS to repair concrete (2 mins)

General Outlook (Harsh, Da, Phil) (5 mins):

Starting with bioreactor
Transformation into spores
Transportation
Spraying on building
Germination
sensing crack + non-environmental kill switch
swarming
end of crack signalling = QS
Filamentous cells
CaCO3
Glue
Kill Switch

BioBrick Discussion (Alan, Younus, Steve) (8 mins altogether; 2 mins each):

Swarming
Subtillin
Filamentous cells - YneA
CaCO3 - Urease

(5 mins remaining)

Whats next... 'Save the babies!!!'

Minideadlines:

Cloning Strategies
- YneA
- One sequence for sfp primers and coding sequence ready (Goksel)
- Subtilin just immunity
Finish Wiki updates Sunday 18th
Team page on Wiki:
- Add to list of advisors: Colin Harwood, Colin Daviy, Wendy Smith and Goksel Misrili
- Deena, Phil and Younus to write about yourself + pictures

Action Points

Da to do everything, thanks

@@ Line 4: / Line 4: @@
 There are 8 cloning strategies which are required:
-*SR1 - finished!
+*'''SR1''' - finished!
-*YneA
+*'''YneA''' - biobrick nearly coming back
-*LacI
+*'''LacI''' - not sure how to test this biobrick
-*2 for Swarming (sfp and swrA) - nearly finished?
+*'''2 for Swarming''' (sfp and swrA) - cloning strategies nearly finished
-*2 for Subtilin (immunity and production) - nearly finished?
+*'''2 for Subtilin''' (immunity and production) - cloning strategies nearly finished
-*RocF (need to use Gibson method) - not started
+*'''RocF''' (need to use Gibson method) - not started
 So only the cloning strategy for SR1 is finished. 7 more to go!
-The swarming and subtilin cloning strategies are nearly finished, YneA nearly coming back and LacI brick not sure how to test.
-Why is testing LacI hard?
 *YneA - our most important BB - Phil, Rach, Deena, Younus