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Team:TU Delft/8 July 2010 content
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| - | + | ==Lab work== | |
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| + | <h4>Ordered DNA stocks</h4> | ||
We harvested the 200 mL bacterial cells of the [https://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 16 DNA parts]. We used 3 mL of the bacterial cells to make [[Team:TU_Delft/protocols/freezing_bacterial_stocks|-80 °C stocks]]. With the rest we centrifuged at 4,000 rmp for 15 minutes and stored the pellets in the -20 °C. | We harvested the 200 mL bacterial cells of the [https://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 16 DNA parts]. We used 3 mL of the bacterial cells to make [[Team:TU_Delft/protocols/freezing_bacterial_stocks|-80 °C stocks]]. With the rest we centrifuged at 4,000 rmp for 15 minutes and stored the pellets in the -20 °C. | ||
| + | <h4>Characterization of Anderson RBS sequences</h4> | ||
| + | [https://2010.igem.org/Team:TU_Delft#/blog?blog=6_July_2010 Tuesday's] ligation products containing I13401 and the 5 Anderson RBS plasmids (J61100, J61101, J61107, J61117, J61127) yielded successful [https://2010.igem.org/Team:TU_Delft/protocols/transformation transformants!] The single colony PCRs performed indicated the presence of inserts with the proper lengths. These colonies were further plated out to yield reincultures for subsequent characterization. | ||
| - | < | + | <h4>Alkane degradation</h4> |
| - | + | Today we got the Gas Chromatograph working, we could identify several peaks. Now we are ready for the real experiments! | |
| - | + | [[Image:Team_TU_Delft_GCtest.PNG|300px|thumb|left]] | |
Revision as of 18:48, 17 July 2010
Contents |
Lab work
Ordered DNA stocks
We harvested the 200 mL bacterial cells of the 16 DNA parts. We used 3 mL of the bacterial cells to make -80 °C stocks. With the rest we centrifuged at 4,000 rmp for 15 minutes and stored the pellets in the -20 °C.
Characterization of Anderson RBS sequences
Tuesday's ligation products containing I13401 and the 5 Anderson RBS plasmids (J61100, J61101, J61107, J61117, J61127) yielded successful transformants! The single colony PCRs performed indicated the presence of inserts with the proper lengths. These colonies were further plated out to yield reincultures for subsequent characterization.
Alkane degradation
Today we got the Gas Chromatograph working, we could identify several peaks. Now we are ready for the real experiments!
