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Team:TU Delft/6 July 2010 content
From 2010.igem.org
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1. AlkB2 on 100 μg/mL Ampicillin | 1. AlkB2 on 100 μg/mL Ampicillin | ||
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2. rubA3 on 100 μg/mL Ampicillin | 2. rubA3 on 100 μg/mL Ampicillin | ||
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3. rubA4 on 100 μg/mL Ampicillin | 3. rubA4 on 100 μg/mL Ampicillin | ||
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4. rubB on 50 μg/mL Kanamycin | 4. rubB on 50 μg/mL Kanamycin | ||
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5. ladA on 100 μg/mL Ampicillin | 5. ladA on 100 μg/mL Ampicillin | ||
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6. ADH on 100 μg/mL Ampicillin | 6. ADH on 100 μg/mL Ampicillin | ||
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7. ALDH on 50 μg/mL Kanamycin | 7. ALDH on 50 μg/mL Kanamycin | ||
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8. bbc1 on 100 μg/mL Ampicillin | 8. bbc1 on 100 μg/mL Ampicillin | ||
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9. AlnA on 100 μg/mL Ampicillin | 9. AlnA on 100 μg/mL Ampicillin | ||
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10. OprG on 100 μg/mL Ampicillin | 10. OprG on 100 μg/mL Ampicillin | ||
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11. PalkS1-2 on 100 μg/mL Ampicillin | 11. PalkS1-2 on 100 μg/mL Ampicillin | ||
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12. PalkB on 100 μg/mL Ampicillin | 12. PalkB on 100 μg/mL Ampicillin | ||
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13. P(CaiF) on 100 μg/mL Ampenicillin | 13. P(CaiF) on 100 μg/mL Ampenicillin | ||
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14. AlkS on 50 μg/mL Kanamycin | 14. AlkS on 50 μg/mL Kanamycin | ||
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15. PhPFD-α on 100 μg/mL Ampicillin | 15. PhPFD-α on 100 μg/mL Ampicillin | ||
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16. PhPFD-β on 100 μg/mL Ampicillin | 16. PhPFD-β on 100 μg/mL Ampicillin | ||
Revision as of 19:09, 12 July 2010
Lab work
The DNA from Mr Gene has finally arrived, now we're ready to build some biobricks!
We were so excited; we immediately solved the DNA in water and performed a transformation with 100 ng of the DNA and plated on the LB agar containing the appropriate antibiotic.
1. AlkB2 on 100 μg/mL Ampicillin
2. rubA3 on 100 μg/mL Ampicillin
3. rubA4 on 100 μg/mL Ampicillin
4. rubB on 50 μg/mL Kanamycin
5. ladA on 100 μg/mL Ampicillin
6. ADH on 100 μg/mL Ampicillin
7. ALDH on 50 μg/mL Kanamycin
8. bbc1 on 100 μg/mL Ampicillin
9. AlnA on 100 μg/mL Ampicillin
10. OprG on 100 μg/mL Ampicillin
11. PalkS1-2 on 100 μg/mL Ampicillin
12. PalkB on 100 μg/mL Ampicillin
13. P(CaiF) on 100 μg/mL Ampenicillin
14. AlkS on 50 μg/mL Kanamycin
15. PhPFD-α on 100 μg/mL Ampicillin
16. PhPFD-β on 100 μg/mL Ampicillin
Pieter is testing different condition for the emulsifying Assay. To determine the emulsifying activity we set up a calibration curve with SDS. He prepared the following samples:
| Hexane (mL) | Tris Buffer pH 8 (mL) | 10% SDS (mL) | |
| B | 1 | 1.1 | 0 |
| 100 | 1 | 1 | 0.1 |
| 50 | 1 | 1.05 | 0.05 |
| 25 | 1 | 1.075 | 0.025 |
| 10 | 1 | 1.09 | 0.01 |
| 5 | 1 | 1.095 | 0.005 |
Kampioenen!!!!
Today the Dutch team will play the semi-final of the World Cup! Hear us cheer! Stop sound
