Team:Newcastle/Meetings/12 July 2010

From 2010.igem.org

(Difference between revisions)

Revision as of 15:32, 12 July 2010

Chair: Da, Minutes: Younus, Comp

. Cloning Strategies

SR1 - Finished
RocF not started - need to use Gibson method;
YneA
2 for Swarming (sfp and swrA)
LacI
2 for Subtilin (Immunity and Production)

Only finished one, SR1. 7 more to go!

Next: swarming and subtilin nearly finished, YneA nearly coming back and LacI brick not sure how to test. Why is testing LacI hard?

YneA - our most important BB - Phil, Rach, Deena, Younus
Swarming (2) - Harsh, Alan, Steve, Da
LacI - Phil, Younus, Rachel
Subtilin (2) - Harsh, Alan, Steve, Deena
RocF - Harsh, Alan, Steve, Da

5 parts to a Cloning Strategy

Purpose and justification
Modelling
Construction
Cloning and Integration
Testing and Characterising

. Wiki

Labbook

. Aim
. Materials and Methods
. Procedure/Protocol
. Results
. Conclusion

Everyone needs to carry out this protocol above Photographs add and label them In charge of wiki - Deena and Rachel (everyone everyday they have to send the links to both of them to check)

20th July - UK iGEM Gathering at Newcastle!

PRESENTATION Introduction - Rach, Deena, Jannetta

Cover Page
Picture of standing us up
Introduction of our iGEM project - using BS to repair concrete (2 mins)

General Outlook (5 mins):

Starting with bioreactor
Transformation into spores
Transportation
Spraying on building
Germination
sensing crack + non-environmental kill switch
swarming
end of crack signalling = QS
Filamentous cells
CaCO3
Glue
Kill Switch

BioBrick Discussion (8 mins altogether; 2 mins each):

Swarming
Subtillin
Filamentous cells - YneA
CaCo3 - Urease

(5 mins remaining)

Whats next... 'Save the babies!!!'

@@ Line 41: / Line 41: @@
 '''20th July - UK iGEM Gathering at Newcastle!'''
-#'''Presentation'''
+'''PRESENTATION'''
 '''Introduction''' - Rach, Deena, Jannetta
 *Cover Page