Team:Slovenia/METHODS and PARTS/notebook/carot
From 2010.igem.org
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<p><span style="font-family: Arial, sans-serif;">6/7/2010 –6/13/2010</span></p> | <p><span style="font-family: Arial, sans-serif;">6/7/2010 –6/13/2010</span></p> | ||
<p> </p> | <p> </p> | ||
- | <p><span style="font-family: Arial, sans-serif;">The following parts from registry were transformed into E. coli DH5α:<br> [http://partsregistry.org/wiki/index.php/Part:BBa_K152005 BBa_K152005], [http://partsregistry.org/wiki/index.php/Part:BBa_K118000 BBa_K118000],[http://partsregistry.org/wiki/index.php/Part:BBa_K118002 BBa_K118002],[http://partsregistry.org/wiki/index.php/Part:BBa_K118003 BBa_K118003],[http://partsregistry.org/wiki/index.php/Part:BBa_K118008 BBa_K118008],[http://partsregistry.org/wiki/index.php/Part:BBa_K118001 BBa_K118001],<br> [http://partsregistry.org/wiki/index.php/Part:BBa_K118004 BBa_K118004],[http://partsregistry.org/wiki/index.php/Part:BBa_K118007 BBa_K118007],[http://partsregistry.org/wiki/index.php/Part:BBa_K118014 BBa_K118014],[http://partsregistry.org/wiki/index.php/Part:BBa_I742158 BBa_I742158] .<br> Plasmids were isolated from 10 mL overnight culture and analyzed by control restriction with enzymes EcoRI and PstI. Plasmid pBAD crtEBIY GFP was constructed from BioBrick [http://partsregistry.org/wiki/index.php/Part:BBa_K118005 BBa_K118005] and pBAD/araC fragment.</span></p> | + | <p><span style="font-family: Arial, sans-serif;">The following parts from registry were transformed into E. coli DH5α:<br> [http://partsregistry.org/wiki/index.php/Part:BBa_K152005 BBa_K152005], [http://partsregistry.org/wiki/index.php/Part:BBa_K118000 BBa_K118000], [http://partsregistry.org/wiki/index.php/Part:BBa_K118002 BBa_K118002],[http://partsregistry.org/wiki/index.php/Part:BBa_K118003 BBa_K118003], [http://partsregistry.org/wiki/index.php/Part:BBa_K118008 BBa_K118008], [http://partsregistry.org/wiki/index.php/Part:BBa_K118001 BBa_K118001],<br> [http://partsregistry.org/wiki/index.php/Part:BBa_K118004 BBa_K118004], [http://partsregistry.org/wiki/index.php/Part:BBa_K118007 BBa_K118007], [http://partsregistry.org/wiki/index.php/Part:BBa_K118014 BBa_K118014],[http://partsregistry.org/wiki/index.php/Part:BBa_I742158 BBa_I742158] .<br> Plasmids were isolated from 10 mL overnight culture and analyzed by control restriction with enzymes EcoRI and PstI. Plasmid pBAD crtEBIY GFP was constructed from BioBrick [http://partsregistry.org/wiki/index.php/Part:BBa_K118005 BBa_K118005] and pBAD/araC fragment.</span></p> |
</div> | </div> | ||
Latest revision as of 22:21, 27 October 2010
week 1week 11
week 2week 12
week 3week 13
week 4week 14
week 5week 15
week 6week 16
week 7week 17
week 8
week 9
week 10
6/7/2010 –6/13/2010
The following parts from registry were transformed into E. coli DH5α:
[http://partsregistry.org/wiki/index.php/Part:BBa_K152005 BBa_K152005], [http://partsregistry.org/wiki/index.php/Part:BBa_K118000 BBa_K118000], [http://partsregistry.org/wiki/index.php/Part:BBa_K118002 BBa_K118002],[http://partsregistry.org/wiki/index.php/Part:BBa_K118003 BBa_K118003], [http://partsregistry.org/wiki/index.php/Part:BBa_K118008 BBa_K118008], [http://partsregistry.org/wiki/index.php/Part:BBa_K118001 BBa_K118001],
[http://partsregistry.org/wiki/index.php/Part:BBa_K118004 BBa_K118004], [http://partsregistry.org/wiki/index.php/Part:BBa_K118007 BBa_K118007], [http://partsregistry.org/wiki/index.php/Part:BBa_K118014 BBa_K118014],[http://partsregistry.org/wiki/index.php/Part:BBa_I742158 BBa_I742158] .
Plasmids were isolated from 10 mL overnight culture and analyzed by control restriction with enzymes EcoRI and PstI. Plasmid pBAD crtEBIY GFP was constructed from BioBrick [http://partsregistry.org/wiki/index.php/Part:BBa_K118005 BBa_K118005] and pBAD/araC fragment.