Team:Michigan/Quorum Sensing

From 2010.igem.org

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~3.5 hrs work
~3.5 hrs work
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''Marc, Ann, Audra and Katie - in Lin Lab''
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Performed biobrick transformations using heat shock
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After second washing:
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*Competent Cell dilution: 1/100
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*ODs for sample 1 and 2 were 0.378 and 0.358 respectively

Revision as of 17:04, 12 July 2010


Michigan Header




Sunday Monday Tuesday Wednesday Thursday Friday Saturday
Week 1 - - - - - - -
Week 2 - - - 7/7/2010 7/8/2010 - -
Week 3 - - - - - - -

Quorum Sensing Team

Members include Alex Pyden, Marcus Lehr, Jennifer Hong, Eric Raynal, Katie Miskovich, and Audra Williams

7/7/10

Jennifer and Alex - in Lin Lab

Made CaCl2 stock solution: 0.1M - 50mL

  • molar mass = 111 g/mol
  • 0.1 mol/L)(.05 L)(111 g/mol) = 555mg
  • dissolved 555 mg CaCl2 in 50mL DIwater
  • vacuum filtered into 50mL tube

Made ampicillin stock solution:

  • 1 g ampicillin
  • 5mL DIwater
  • ethanol
  • filtered by syringe into 15mL tube
  • put into ten 1mL alliquots in 1.5mL tubes
  • stored in -20°C in ERB lab

Inoculated E. coli DH5α from frozen stock into 12 mL LB broth

  • put in 30°C, 200 rpm shaking, overnight

~1.5 hrs

7/8/10

Alex, Jennifer and Eric - in ERB

Made LB agar plates w/ ampicillin

  • 10 g LB broth
  • 7.5 g agar
  • 500 mL DI water
  • autoclaved mixture at 121°C for 30 min (sterilize time), following Mike Nelson's protocol

Created protocols for Obtaining Deionized Water in the ERB and ERB Spectrophotometer

poured 20 LB-amp plates (large)

  • left to solidify in ERB 1230
  • 12 plates were used by Ann for biobrick transformations
  • 8 plates were stored in 4°C ERB

~3.5 hrs work

Marc, Ann, Audra and Katie - in Lin Lab

Performed biobrick transformations using heat shock

After second washing:

  • Competent Cell dilution: 1/100
  • ODs for sample 1 and 2 were 0.378 and 0.358 respectively
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