"
Team:USTC/Project/shell/result
From 2010.igem.org
(Difference between revisions)
| Line 23: | Line 23: | ||
</html> | </html> | ||
| - | + | In the expression of protein, we use and unite the method mentioned in the previous papers. [see protocol] The bacterial was induced by 1 mM IPTG at 30 ℃ for 4 hours. The results was as follows.[the one without pduN was a negative control used to prove the function of pduN: in the growth process, the one without pduN tends to grow with more deposit implying its possible role in the vertex] | |
[[Image:Pdu_iptg.jpg| SDS-PAGE of pdu proteins]] | [[Image:Pdu_iptg.jpg| SDS-PAGE of pdu proteins]] | ||
| + | |||
| + | We use the ultracentrifuge to purify the proteins, with 48000 g speed, we get our targeted proteins in the pellet. | ||
[[Image:Pdu_Sds-page.jpg| SDS-PAGE of pdu proteins]] | [[Image:Pdu_Sds-page.jpg| SDS-PAGE of pdu proteins]] | ||
Revision as of 03:32, 28 October 2010
In the expression of protein, we use and unite the method mentioned in the previous papers. [see protocol] The bacterial was induced by 1 mM IPTG at 30 ℃ for 4 hours. The results was as follows.[the one without pduN was a negative control used to prove the function of pduN: in the growth process, the one without pduN tends to grow with more deposit implying its possible role in the vertex]
We use the ultracentrifuge to purify the proteins, with 48000 g speed, we get our targeted proteins in the pellet.
