From 2010.igem.org
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| - | | [[Image:MG_unipv10_efficiency.jpg|300px|thumb|center|MG1655 plate (with satellite colonies)]] || [[Image:BW25141_unipv10_efficiency.jpg|300px|thumb|center|BW25141 plate]] | + | | [[Image:MG_unipv10_efficiency.jpg|200px|thumb|center|MG1655 plate (with satellite colonies)]] || [[Image:BW25141_unipv10_efficiency.jpg|200px|thumb|center|BW25141 plate]] || [[Image:BW25142_unipv10_efficiency.jpg|200px|thumb|center|BW25142 plate]] |
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| - | | [[Image:BW25142_unipv10_efficiency.jpg|300px|thumb|center|BW25142 plate]] || [[Image:BW53474_unipv10_efficiency.jpg|300px|thumb|center|BW53474 plate]] | + | |[[Image:BW53474_unipv10_efficiency.jpg|200px|thumb|center|BW53474 plate]] || [[Image:DH5-alpha_unipv10_efficiency.jpg|200px|thumb|center|DH5-alpha plate]] |
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| - | |colspan="2"|[[Image:DH5-alpha_unipv10_efficiency.jpg|300px|thumb|center|DH5-alpha plate]]
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Revision as of 21:10, 21 July 2010
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JULY: WEEK 2
July, 5th
July, 6th
July, 7th
July, 8th
Transformation of 1ul (~4ng) of miniprepped <partinfo>BBa_J23118</partinfo> into 100ul of home-made competent cells
- MG1655
- BW25141
- BW25142
- BW53474
- DH5-alpha (as control)
to verify the transformation efficiency.
Transformed bacteria were plated on LB+Amp and left overnight in oven, 37°C.
July, 9th
We checked the presence of colonies in plates.
All plates showed red colonies!
 MG1655 plate (with satellite colonies) | |
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We calculated (thanks Nicolò) efficiency as #colonies/ug DNA plated
| Strain | #colonies | Efficiency
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| MG1655 | 379 | ~10^5
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| BW25141 | 1431 | ~10^6
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| BW25142 | 2374 | ~10^6
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| BW53474 | 9984 | ~10^7
(very difficult to count correctly)
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| DH5alpha | 7475
(only 100ul of cells plated) | ~ 10^7
(very difficult to count correctly,
previous tests showed an efficiency of ~10^8)
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"
