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Team:NYMU-Taipei
From 2010.igem.org
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*'''<font size=3>Why do we want to do that?</font><br> | *'''<font size=3>Why do we want to do that?</font><br> | ||
There are already many genetic parts in the Biobrick Parts Registry and the numbers are growing rapidly. Every year every igem teams will build one or more circuits based on the parts at partsregistry. But where are the design rules to put these parts into circuits of devices and systems? Apparently, the "Assembly Standards" listed at the partsregistry are only used to connect compatible restriction enzyme cutting sites. They are NOT designing principles. Our iGEM team is very interested in the detailed design rules played in the central dogma; especially those principles connect mRNA translation to protein folding. Traditionally, we know about the circuits we made are working or not by the expression of reporter genes. But now we want to quantitative description of gene expression in both space and time. For the above reasons, we must to be speed up the experiment for researching the more rules. <br> | There are already many genetic parts in the Biobrick Parts Registry and the numbers are growing rapidly. Every year every igem teams will build one or more circuits based on the parts at partsregistry. But where are the design rules to put these parts into circuits of devices and systems? Apparently, the "Assembly Standards" listed at the partsregistry are only used to connect compatible restriction enzyme cutting sites. They are NOT designing principles. Our iGEM team is very interested in the detailed design rules played in the central dogma; especially those principles connect mRNA translation to protein folding. Traditionally, we know about the circuits we made are working or not by the expression of reporter genes. But now we want to quantitative description of gene expression in both space and time. For the above reasons, we must to be speed up the experiment for researching the more rules. <br> | ||
| - | *'''<font size=3>Specific aims:</font>'''<br> | + | *'''<font size=3>Specific aims of our device design and engineering:</font>'''<br> |
| - | ** | + | ** detect gene expression quantitatively in both space and time. |
| - | ** | + | ** specific insight into the flow of genetic information. |
| - | ** | + | ** provide speedy ways to report and stop gene expression. |
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[[Image:Nymusyb.png|500px]] | [[Image:Nymusyb.png|500px]] | ||
Revision as of 18:10, 27 October 2010
| Home | Project Overview | Speedy reporter | Speedy switch | Speedy protein degrader | Experiments and Parts | Applications | F.A.Q | About Us |
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SpeedyBac
Provide a faster assay system for exploring the design rules of synthetic biology.
There are already many genetic parts in the Biobrick Parts Registry and the numbers are growing rapidly. Every year every igem teams will build one or more circuits based on the parts at partsregistry. But where are the design rules to put these parts into circuits of devices and systems? Apparently, the "Assembly Standards" listed at the partsregistry are only used to connect compatible restriction enzyme cutting sites. They are NOT designing principles. Our iGEM team is very interested in the detailed design rules played in the central dogma; especially those principles connect mRNA translation to protein folding. Traditionally, we know about the circuits we made are working or not by the expression of reporter genes. But now we want to quantitative description of gene expression in both space and time. For the above reasons, we must to be speed up the experiment for researching the more rules.
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To achieve our specific aim, we have designed a novel reporting device (Speedy reporter) for quickly detectin and measuring the mRNA location and quantity, it can also be used for protein detection. And we design a novel switch (Speedy switch) for control the mRNA translation of gene expression. We have also designed a faster degradation device (Speedy protein degrader); it allows us to regulate the degradation time for studying the mRNAs without the interference from translation and quickly stopping the gene expression.
The official web pages of our school - National Yang Ming University (NYMU):
Click the following two links to see The Beauty of NYMU
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