Team:HokkaidoU Japan/Notebook/September29
From 2010.igem.org
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*erectrophoresed the samples.Marker is 10 uL λ/HindIII EcoRI. | *erectrophoresed the samples.Marker is 10 uL λ/HindIII EcoRI. | ||
+ | [[Image:HokkaidoU Japan 20100929b.jpg|200px|left|thumb|Electrophoresis of colony PCR sample]] | ||
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+ | = Cultivation of colony = | ||
- | * | + | *cultivated the colonies which were used for PCR.Each Samples were cultured in LB contained Ampicillin and 20% Arabinose or contained only Ampicillin. |
Revision as of 16:32, 27 October 2010
Electrophoresis of Yesterday Samples
- electrophoresed 6 uL Marker λ/HindIII EcoRI and 1 uL of two yesterday samples.T3SS signal wasn't observed.
Colony PCR of Arabinose Promoter + RBS + GFP + double terminator
- PCRed 5 colony.
PCR mix
Reagent | Amount |
---|---|
Quick Taq | 25 uL |
EX-F | 0.5 uL |
PS-R | 0.5 uL |
Total | 26 uL |
- PCRed according to the table below.98C and 68C for 35 cycles.
temp | time |
---|---|
94C | 2 min |
94C | 30 sec |
68C | 90 sec |
4C | hold |
- erectrophoresed the samples.Marker is 10 uL λ/HindIII EcoRI.
Cultivation of colony
- cultivated the colonies which were used for PCR.Each Samples were cultured in LB contained Ampicillin and 20% Arabinose or contained only Ampicillin.