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Team:HokkaidoU Japan/Notebook/September29
From 2010.igem.org
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*electrophoresed 6 uL Marker λ/HindIII EcoRI and 1 uL of two yesterday samples.T3SS signal wasn't observed. | *electrophoresed 6 uL Marker λ/HindIII EcoRI and 1 uL of two yesterday samples.T3SS signal wasn't observed. | ||
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| + | [[Image:HokkaidoU Japan 20100929b.JPG|200px|left|thumb|Electrophoresis of colony PCR sample]] | ||
*erectrophoresed the samples.Marker is 10 uL λ/HindIII EcoRI. | *erectrophoresed the samples.Marker is 10 uL λ/HindIII EcoRI. | ||
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Revision as of 16:21, 27 October 2010
since 13 Jul 2010
since 1 Aug 2010
Electrophoresis of Yesterday Samples
- electrophoresed 6 uL Marker λ/HindIII EcoRI and 1 uL of two yesterday samples.T3SS signal wasn't observed.
Colony PCR of Arabinose Promoter + RBS + GFP + double terminator
- PCRed 5 colony.
PCR mix
| Reagent | Amount |
|---|---|
| Quick Taq | 25 uL |
| EX-F | 0.5 uL |
| PS-R | 0.5 uL |
| Total | 26 uL |
- PCRed according to the table below.98C and 68C for 35 cycles.
| temp | time |
|---|---|
| 94C | 2 min |
| 94C | 30 sec |
| 68C | 90 sec |
| 4C | hold |
File:HokkaidoU Japan 20100929b.JPG
Electrophoresis of colony PCR sample
- erectrophoresed the samples.Marker is 10 uL λ/HindIII EcoRI.
