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Team:TU Delft/11 June 2010 content
From 2010.igem.org
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Today we [[Team:TU_Delft/protocols/mini-prep_plasmid_isolation|isolated]] a mass stock of all the plasmids we will be using for BioBrick assembly. Next to that we also started digesting the first parts we will be characterizing. | Today we [[Team:TU_Delft/protocols/mini-prep_plasmid_isolation|isolated]] a mass stock of all the plasmids we will be using for BioBrick assembly. Next to that we also started digesting the first parts we will be characterizing. | ||
| + | |||
| + | The following plasmid concentrations were obtained: | ||
| + | |||
| + | {| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1" | ||
| + | |BioBrick | ||
| + | |Concentration (ng/μL) | ||
| + | |- | ||
| + | |pSB3T5 | ||
| + | |266.0 | ||
| + | |- | ||
| + | |pSB1T3 | ||
| + | |414.0 | ||
| + | |- | ||
| + | |pSB1C3 | ||
| + | |284.7 | ||
| + | |- | ||
| + | |pSB3C5 | ||
| + | |329.3 | ||
| + | |- | ||
| + | |pSB1K3 | ||
| + | |270.1 | ||
| + | |- | ||
| + | |pSB1A3 | ||
| + | |271.9 | ||
| + | |- | ||
| + | |I13401 | ||
| + | |232.1 | ||
| + | |} | ||
[[Image:P1010002_small.jpg|250px|thumb|left|Ramon & Hugo in the lab]] | [[Image:P1010002_small.jpg|250px|thumb|left|Ramon & Hugo in the lab]] | ||
Revision as of 12:01, 13 July 2010
Lab work
Today we isolated a mass stock of all the plasmids we will be using for BioBrick assembly. Next to that we also started digesting the first parts we will be characterizing.
The following plasmid concentrations were obtained:
| BioBrick | Concentration (ng/μL) |
| pSB3T5 | 266.0 |
| pSB1T3 | 414.0 |
| pSB1C3 | 284.7 |
| pSB3C5 | 329.3 |
| pSB1K3 | 270.1 |
| pSB1A3 | 271.9 |
| I13401 | 232.1 |
Spring Workshop
Almost the whole team left for the iGEM spring workshop in Évry, Paris. The drive for some of us was a bit laborious (traffic jams at the middle of the night/closed roads etc.), and others actually watched the World Cup soccer match... But in the end we all arrived at the hotel in Évry little before 2 AM, Saturday morning, and got some well deserved sleep.
