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Team:Harvard/parts
From 2010.igem.org
(Difference between revisions)
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<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">link</a></td></tr> | <tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>-</td><td><a href="https://static.igem.org/mediawiki/2010/8/82/Ger.txt">link</a></td></tr> | ||
| - | <tr><td>F4</td><td>BBa_K382020</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr> | + | <tr><td>F4</td><td>BBa_K382020</td><td><i>Arabidopsis</i>-optimized Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr> |
| - | <tr><td>F5</td><td>BBa_K382021</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr> | + | <tr><td>F5</td><td>BBa_K382021</td><td><i>Arabidopsis</i>-optimized Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr> |
<tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr> | <tr><td>F1</td><td>BBa_K382022</td><td>pENTCUP2 promoter</td><td>Plant specific promoter. Used to drive expression of our flavor constructs in plants. </td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382022">link</a></td></tr> | ||
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<tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr> | <tr><td>F3</td><td>BBa_K382024</td><td>NOSt terminator + stop</td><td>Plant specific terminator. Terminates transcription with stop codon on 5' end.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382024">link</a></td></tr> | ||
| - | <tr><td></td><td>BBa_K382025</td><td>Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr> | + | <tr><td></td><td>BBa_K382025</td><td><i>Arabidopsis</i>-optimized Miraculin StrepII N-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382025">link</a></td></tr> |
| - | <tr><td></td><td>BBa_K382026</td><td>Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr> | + | <tr><td></td><td>BBa_K382026</td><td><i>Arabidopsis</i>-optimized Brazzein StrepII N-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382026">link</a></td></tr> |
| - | <tr><td>F9</td><td>BBa_K382027</td><td>Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr> | + | <tr><td>F9</td><td>BBa_K382027</td><td><i>Arabidopsis</i>-optimized Miraculin StrepII C-terminus</td><td>Miraculin with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382027">link</a></td></tr> |
| - | <tr><td>F8</td><td>BBa_K382028</td><td>Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr> | + | <tr><td>F8</td><td>BBa_K382028</td><td><i>Arabidopsis</i>-optimized Brazzein StrepII C-terminus</td><td>Brazzein with StrepII tag on C-terminus for Western Blot.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382028">link</a></td></tr> |
<tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr> | <tr><td>GF5</td><td>BBa_K382030</td><td>RXRHm</td><td>Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f5">link</a></td></tr> | ||
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<tr><tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>amp</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr> | <tr><tr><td></td><td>BBa_K382037</td><td>Ecdysone Receptor</td><td> Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide </td><td>amp</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/f7">link</a></td></tr> | ||
| - | <tr><td>F6</td><td>BBa_K382040</td><td>Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr> | + | <tr><td>F6</td><td>BBa_K382040</td><td><i>Arabidopsis</i>-optimized Brazzein YFP2x C-terminus</td><td>Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F6">link</a></td></tr> |
| - | <tr><td>F7</td><td>BBa_K382041</td><td>Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F7">link</a></td></tr> | + | <tr><td>F7</td><td>BBa_K382041</td><td><i>Arabidopsis</i>-optimized Miraculin YFP2x C-terminus</td><td>Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression</td><td>chloramphenicol</td><td>-</td><td><a href="https://2010.igem.org/Team:Harvard/F7">link</a></td></tr> |
| - | <tr><td></td><td>BBa_K382050</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan</td><td>pat</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr> | + | <tr><td></td><td>BBa_K382050</td><td><i>Arabidopsis</i>-optimized Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan</td><td>pat</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr> |
| - | <tr><td></td><td>BBa_K382051</td><td>Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan</td><td>nptII</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr> | + | <tr><td></td><td>BBa_K382051</td><td><i>Arabidopsis</i>-optimized Miraculin</td><td>Taste-inverting protein turns sour to sweet. 191 amino acid sequence</td><td>kan</td><td>nptII</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382021">link</a></td></tr> |
| - | <tr><td></td><td>BBa_K382052</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan</td><td>pat</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr> | + | <tr><td></td><td>BBa_K382052</td><td><i>Arabidopsis</i>-optimized Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan</td><td>pat</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr> |
| - | <tr><td></td><td>BBa_K382053</td><td>Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan</td><td>nptII</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr> | + | <tr><td></td><td>BBa_K382053</td><td><i>Arabidopsis</i>-optimized Brazzein</td><td>Sweet-tasting protein. 54 amino acid sequence. </td><td>kan</td><td>nptII</td><td><a href="https://2010.igem.org/Team:Harvard/BBa_K382020">link</a></td></tr> |
<tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan</td><td>pat</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr> | <tr><td></td><td>BBa_K382054</td><td>amiRNA GFP</td><td>amiRNA construct for GFP knockdown</td><td>kan</td><td>pat</td><td><a href="https://2010.igem.org/Team:Harvard/allergy/AmirnaGFP.txt">link</a></td></tr> | ||
Revision as of 13:56, 27 October 2010
parts
| name | biobrick name | original name | description | resistance (bacteria) | resistance (plants) | sequence |
| V7 | BBa_K382000 | pORE Open Series Vector with BioBrick Sites | Agrobacterium vector open series with biobrick cloning site | kan | pat | link |
| V8 | BBa_K382001 | pORE Open Series Vector with BioBrick Sites | Agrobacterium vector open series with biobrick cloning site | kan | nptII | link |
| V9 | BBa_K382002 | pORE Expression Series Vector with BioBrick Sites | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | kan | pat | link |
| V10 | BBa_K382003 | pORE Expression Series Vector with BioBrick Sites | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | kan | nptII | link |
| V11 | BBa_K382004 | pORE Reporter Series Vector with BioBrick Sites | Agrobacterium vector with gusA reporter and biobrick cloning site | kan | nptII | link |
| V12 | BBa_K382005 | pORE Reporter Series Vector with BioBrick Sites | Agrobacterium vector with smgfp reporter and biobrick cloning site | kan | nptII | link |
| C1 | BBa_K382010 | amiRNA LUT2 | amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown | chloramphenicol | - | link |
| C2 | BBa_K382011 | amiRNA BETA-OHASE 1 | amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown | chloramphenicol | - | link |
| C3 | BBa_K382012 | amiRNA LYC | amiRNA construct for lycopene beta cyclase (LYC) knockdown | chloramphenicol | - | link |
| A3 | BBa_K382014 | ihpRNA Bet | Intron hairpin Bet Knockdown construct | amp | - | link |
| A4 | BBa_K382015 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | chloramphenicol | - | link |
| A2 | BBa_K382017 | amiRNA LTP | LTP knockdown construct | amp | - | link |
| A4 | BBa_K382018 | PDK intron hairpin | Intron hairpin construct | amp | - | link |
| A5 | BBa_K382019 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | chloramphenicol | - | link |
| F4 | BBa_K382020 | Arabidopsis-optimized Brazzein | Sweet-tasting protein. 54 amino acid sequence. | chloramphenicol | - | link |
| F5 | BBa_K382021 | Arabidopsis-optimized Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence | chloramphenicol | - | link |
| F1 | BBa_K382022 | pENTCUP2 promoter | Plant specific promoter. Used to drive expression of our flavor constructs in plants. | chloramphenicol | - | link |
| F2 | BBa_K382023 | NOSt terminator | Plant specific terminator. Terminates transcription but no stop codon on end. | chloramphenicol | - | link |
| F3 | BBa_K382024 | NOSt terminator + stop | Plant specific terminator. Terminates transcription with stop codon on 5' end. | chloramphenicol | - | link |
| BBa_K382025 | Arabidopsis-optimized Miraculin StrepII N-terminus | Miraculin with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | link | |
| BBa_K382026 | Arabidopsis-optimized Brazzein StrepII N-terminus | Brazzein with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | link | |
| F9 | BBa_K382027 | Arabidopsis-optimized Miraculin StrepII C-terminus | Miraculin with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | link |
| F8 | BBa_K382028 | Arabidopsis-optimized Brazzein StrepII C-terminus | Brazzein with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | link |
| GF5 | BBa_K382030 | RXRHm | Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide. | chloramphenicol | - | link |
| GF4 | BBa_K382031 | RXRLc | Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide | amp | - | link |
| BBa_K382033 | Ecdysone Receptor | Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide | chloramphenicol | - | link | |
| BBa_K382034 | Act2pLacO | Actin promoter with LacO sites | amp | - | link | |
| GF3 | BBa_K382035 | LacIN | Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter | chloramphenicol | - | link |
| GF4 | BBa_K382031 | RXRLc | Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide | amp | - | link |
| GF5 | BBa_K382036 | RXRHm | Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide. | amp | - | link |
| BBa_K382037 | Ecdysone Receptor | Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide | amp | - | link | |
| F6 | BBa_K382040 | Arabidopsis-optimized Brazzein YFP2x C-terminus | Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | link |
| F7 | BBa_K382041 | Arabidopsis-optimized Miraculin YFP2x C-terminus | Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | link |
| BBa_K382050 | Arabidopsis-optimized Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence | kan | pat | link | |
| BBa_K382051 | Arabidopsis-optimized Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence | kan | nptII | link | |
| BBa_K382052 | Arabidopsis-optimized Brazzein | Sweet-tasting protein. 54 amino acid sequence. | kan | pat | link | |
| BBa_K382053 | Arabidopsis-optimized Brazzein | Sweet-tasting protein. 54 amino acid sequence. | kan | nptII | link | |
| BBa_K382054 | amiRNA GFP | amiRNA construct for GFP knockdown | kan | pat | link | |
| BBa_K382055 | amiRNA GFP | amiRNA construct for GFP knockdown | kan | nptII | link | |
| BBa_K382058 | amiRNA LUT2 | amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown | kan | pat | link | |
| BBa_K382059 | amiRNA BETA-OHASE 1 | amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown | kan | pat | link | |
| BBa_K382060 | amiRNA LYC | amiRNA construct for lycopene beta cyclase (LYC) knockdown | kan | pat | link | |
| BBa_K382061 | amiRNA LUT2 | amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown | kan | nptII | link | |
| BBa_K382062 | amiRNA BETA-OHASE 1 | amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown | kan | nptII | link | |
| BBa_K382063 | amiRNA LYC | amiRNA construct for lycopene beta cyclase (LYC) knockdown | kan | nptII | link | |
| BBa_K382064 | amiRNA LTP | LTP knockdown construct | kan | pat | link | |
| BBa_K382065 | amiRNA LTP | LTP knockdown construct | kan | nptII | link | |
| BBa_K382066 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | kan | pat | link | |
| BBa_K382067 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | kan | nptII | link | |
| BBa_K382068 | ihpRNA BetV1 | Intron hairpin Bet Knockdown construct | kan | pat | link | |
| BBa_K382069 | ihpRNA BetV1 | Intron hairpin Bet Knockdown construct | kan | nptII | link | |
| BBa_K382070 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | kan | pat | link | |
| BBa_K382071 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | kan | nptII | link |
