Team:UNIPV-Pavia/Calendar/July/settimana2

From 2010.igem.org

(Difference between revisions)
(July, 8th)
m (July, 8th)
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==July, 8th==
==July, 8th==
-
Transformation of 4ug of miniprepped <partinfo>BBa_J23118</partinfo> into home-made competent cells
+
Transformation of 1ul (~4ng) of miniprepped <partinfo>BBa_J23118</partinfo> into 100ul of home-made competent cells
*MG1655
*MG1655
*BW25141
*BW25141
*BW25142
*BW25142
*BW53474
*BW53474
-
*DH5alpha (as control)
+
*DH5-alpha (as control)
to verify the transformation efficiency.
to verify the transformation efficiency.
-
Transformed bacteria are plated on LB+Amp plates and left ON in oven, 37°C.
+
Transformed bacteria were plated on LB+Amp and left overnight in oven, 37°C.
==July, 9th==
==July, 9th==

Revision as of 12:33, 9 July 2010

JULY: WEEK 2




July, 5th

July, 6th

July, 7th

July, 8th

Transformation of 1ul (~4ng) of miniprepped <partinfo>BBa_J23118</partinfo> into 100ul of home-made competent cells

  • MG1655
  • BW25141
  • BW25142
  • BW53474
  • DH5-alpha (as control)

to verify the transformation efficiency.

Transformed bacteria were plated on LB+Amp and left overnight in oven, 37°C.

July, 9th

Calendar

July

week 1

week 2

week 3

week 4