Team:TzuChiU Formosa/Safety

From 2010.igem.org

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       <h2>Q1. Would any of your project ideas raise  safety issues in terms of: <br />
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       <h2><strong>Q1. Would any of your project ideas raise  safety issues in terms of: <br />
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         researcher safety, public safety, or: environmental safety? </h2>
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         researcher safety, public safety, or: environmental safety? </strong></h2>
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       <h3>We only used <em>E. coli</em> DH5α in all our experiments when required. DH5α  is not pathogenic to humans nor animals and will not survive in the  environment. The inserted sequences encode only plant enzymes which is  necessary to made β-carotene and are highly unlikely to  confer any harmful phenotype on the disabled <em>E.coli</em> host nor animals. Standard  good lab practices were followed strictly in handling all bacterial samples,  including wearing protective groves, working in lamina flow, autoclaving  contaminated waste, bleaching discarded liquid cultures, and washing hands  before and after experiments.</h3>
       <h3>We only used <em>E. coli</em> DH5α in all our experiments when required. DH5α  is not pathogenic to humans nor animals and will not survive in the  environment. The inserted sequences encode only plant enzymes which is  necessary to made β-carotene and are highly unlikely to  confer any harmful phenotype on the disabled <em>E.coli</em> host nor animals. Standard  good lab practices were followed strictly in handling all bacterial samples,  including wearing protective groves, working in lamina flow, autoclaving  contaminated waste, bleaching discarded liquid cultures, and washing hands  before and after experiments.</h3>
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       <h2>Q2. Do any of the new BioBrick parts (or devices)  that you made this year raise any safety issues? If yes, <br />
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       <h2><strong>Q2. Do any of the new BioBrick parts (or devices)  that you made this year raise any safety issues? If yes, <br />
         did you document these issues in the Registry? <br />
         did you document these issues in the Registry? <br />
         how did you manage to handle the safety  issue? <br />
         how did you manage to handle the safety  issue? <br />
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         how could other teams learn from your experience?</h2>
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         how could other teams learn from your experience?</strong></h2>
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       <h3>No.</h3>
       <h3>No.</h3>
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       <h2>Q3. Is there a local biosafety group, committee,  or review board at your institution? <br />
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       <h2><strong>Q3. Is there a local biosafety group, committee,  or review board at your institution? <br />
         If yes, what does your local biosafety group  think about your project? <br />
         If yes, what does your local biosafety group  think about your project? <br />
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         If no, which specific biosafety rules or  guidelines do you have to consider in your country?</h2>
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         If no, which specific biosafety rules or  guidelines do you have to consider in your country?</strong></h2>
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       <h3>Yes. All bacterial or plasmid used have to  obtain an approval from University's environmental and biosafety committees.  Environmental and biosafety officers made regular random visit to each  laboratory to ensure all laboratories are comply with environmental and  biosafety regulations. So far our project has received positive responds from  the committees.</h3>
       <h3>Yes. All bacterial or plasmid used have to  obtain an approval from University's environmental and biosafety committees.  Environmental and biosafety officers made regular random visit to each  laboratory to ensure all laboratories are comply with environmental and  biosafety regulations. So far our project has received positive responds from  the committees.</h3>
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       <h2>Q4. Do you have any other ideas how to deal with  safety issues that could be useful for future iGEM competitions? How could  parts, devices and systems be made even safer through biosafety engineering? </h2>
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       <h2><strong>Q4. Do you have any other ideas how to deal with  safety issues that could be useful for future iGEM competitions? How could  parts, devices and systems be made even safer through biosafety engineering? </strong></h2>
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       <h3>Perhaps parts, devices or systems can be divided  into two categories such as prokaryotes and eukaryotes and regulate under  standard environmental and biosafety regulations.</h3>
       <h3>Perhaps parts, devices or systems can be divided  into two categories such as prokaryotes and eukaryotes and regulate under  standard environmental and biosafety regulations.</h3>
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Revision as of 10:07, 27 October 2010

無標題文件

Safety Q&A

For iGEM 2010 we are asked to detail how we approached any issues of biological safety

associated with their projects (see here). Here are the questions and our answers:

Q1. Would any of your project ideas raise safety issues in terms of:
researcher safety, public safety, or: environmental safety?

We only used E. coli DH5α in all our experiments when required. DH5α is not pathogenic to humans nor animals and will not survive in the environment. The inserted sequences encode only plant enzymes which is necessary to made β-carotene and are highly unlikely to confer any harmful phenotype on the disabled E.coli host nor animals. Standard good lab practices were followed strictly in handling all bacterial samples, including wearing protective groves, working in lamina flow, autoclaving contaminated waste, bleaching discarded liquid cultures, and washing hands before and after experiments.

 

Q2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,
did you document these issues in the Registry?
how did you manage to handle the safety issue?
how could other teams learn from your experience?

No.

Q3. Is there a local biosafety group, committee, or review board at your institution?
If yes, what does your local biosafety group think about your project?
If no, which specific biosafety rules or guidelines do you have to consider in your country?

Yes. All bacterial or plasmid used have to obtain an approval from University's environmental and biosafety committees. Environmental and biosafety officers made regular random visit to each laboratory to ensure all laboratories are comply with environmental and biosafety regulations. So far our project has received positive responds from the committees.

Q4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

Perhaps parts, devices or systems can be divided into two categories such as prokaryotes and eukaryotes and regulate under standard environmental and biosafety regulations.