Team:Johns Hopkins
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*Characterized two full voltage-activated promoters for yeast. | *Characterized two full voltage-activated promoters for yeast. | ||
*Developed a model to describe the transcriptional response to voltage of our system. | *Developed a model to describe the transcriptional response to voltage of our system. | ||
- | *Successfully leveraged post-translational modification machinery, namely phosphorylation of Crz1 by calcineurin, to initiate a transcriptional response. | + | *Successfully leveraged post-translational modification machinery, namely de-phosphorylation of Crz1 by calcineurin, to initiate a transcriptional response. |
*Fabricated a highly accurate microfluidic electro-stimulation setup using gold electrodes on a silicone wafer and are currently in the process of refining the fabrication technique. | *Fabricated a highly accurate microfluidic electro-stimulation setup using gold electrodes on a silicone wafer and are currently in the process of refining the fabrication technique. | ||
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[[Image:IGEM_Team_Photo.jpg|650px|center|thumb| JHU iGEM 2010]] | [[Image:IGEM_Team_Photo.jpg|650px|center|thumb| JHU iGEM 2010]] |
Revision as of 15:52, 27 October 2010
Genetically engineered Saccharomyces cerevisiae that is responsive to voltage signals at a transcriptional level. ' Using our specifically designed CRZ1 binding elements in tandem with fluorescent reporter genes, we characterized calcineurin-CRZ1 mediated calcium response pathway in yeast. In doing so we have taken the first step in creating an interface between cellular systems and computers by allowing cells to respond to voltage signals, the language of computers. Possible applications:
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Team: We are a team of 9 undergraduate students deeply interested in synthetic biology. We hail from a variety of disciplines including, chemistry, biology and engineering. We’re a fresh new team with varying levels of experience united by our passion for science. |
Our Accomplishments
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