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Team:Queens-Canada/9 August 2010
From 2010.igem.org
(New page: <h2>August 9, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Miniprep</h3> Hao, Thai, Nelson, Chris, Yuli '''eCFP'''::1C3, '''NpHR'''::1C3, '''pStr-...) |
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Used 0.7 g agarose in 100 mL TBE. Gel run for 26 mins at 110 V. | Used 0.7 g agarose in 100 mL TBE. Gel run for 26 mins at 110 V. | ||
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| + | <h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3> | ||
| + | Hao | ||
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| + | Practiced injections with Sur-5::GFP marker. | ||
{{:Team:Queens-Canada/foot}} | {{:Team:Queens-Canada/foot}} | ||
Latest revision as of 02:00, 28 October 2010
August 9, 2010
Miniprep
Hao, Thai, Nelson, Chris, Yuli
'eCFP::1C3, NpHR::1C3, pStr-1::1C3, pStr-220::1C3, pCeh-12::1C3, pOsm-10::1C3, pOdr-1::1C3, NpHR::1C3, pSrb-ChR2'::1C3, mCherry::1C3, pSB1C3
'pIC57ChR2'::1C3 did not pull through vacuum very well, may contain genomic DNA.
Gel and Digest
Chris and Thai
pOsm-10, pStr-220, pOdr-1
Cut with EcoRI and PstI.
Used 0.7 g agarose in 100 mL TBE. Gel run for 26 mins at 110 V. Note: [4] pOsm-10 digest had <10 µL of liquid, most likely due to error during master mix loading.
Gel Electrophoresis
Chris and Thai
pOdr-1, NpHR, pStr-1, pCeh-12
Used 0.7 g agarose in 100 mL TBE. Gel run for 33 mins at 110 V.
Gel Electrophoresis
Chris and Thai
pCeh-12, pSrb-6, pIC576, eCFP
Used 0.7 g agarose in 100 mL TBE. Gel run for 26 mins at 110 V.
Microinjection
Hao
Practiced injections with Sur-5::GFP marker.
