"
Team:Queens-Canada/6 August 2010
From 2010.igem.org
(Difference between revisions)
| Line 30: | Line 30: | ||
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | <h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | ||
Hao and Thai | Hao and Thai | ||
| + | '''eYFP''','''ChR2''', '''mCherry''' | ||
Used 0.3 g agarose in 45 mL TBE. Gel run for 45 mins at 100 V. | Used 0.3 g agarose in 45 mL TBE. Gel run for 45 mins at 100 V. | ||
{{:Team:Queens-Canada/foot}} | {{:Team:Queens-Canada/foot}} | ||
Revision as of 01:15, 28 October 2010
August 6, 2010
Gel Electrophoresis
Hao
pSra-10, pHsp-3
Used 0.3 g agarose in 40 mL TBE. Gel run for 50 mins at 100 V.
PCR
Hao and Thai
ChR2, eCFP, eYFP, mCherry
Program run using 9:1 FAST:HIFI blend with an extension time of 15 s and annealing temperature of 61 °C.
mCherry was run for 5 cycles without primers, then for 35 cycles with primers.
Ligation
Hao and Steve and Thai
NpHR, pOdr-10, eYFP, pStr-220, mCherry, pOsm-10, pOdr-1, eCFP, pMec-7, pFlp-1, pCeh-12, pIC518, ChR2, pSrb-6, pSB1C3
All inserts ligated into pSB1C3. T4 ligase used.
Heat Shock
Hao and Thai Ligation products from 06/08/10
Gel Electrophoresis
Hao and Thai eYFP,ChR2, mCherry
Used 0.3 g agarose in 45 mL TBE. Gel run for 45 mins at 100 V.
