Team:Queens-Canada/12 July 2010
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Used 0.7 g agarose in 100 mL TBE. Gel run for 55 mins at 100 V. | Used 0.7 g agarose in 100 mL TBE. Gel run for 55 mins at 100 V. | ||
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+ | <h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3> | ||
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+ | Practiced injections with water. | ||
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Latest revision as of 01:51, 28 October 2010
July 12, 2010
Liquid Culture
Thai
pBSII
PCR
Hao and Basia
pOdr-10, pOdr-1, pSrb-6, pSra-10, pOsm-10, pCeh-12, pFlp-1, pStr-1
Program run using HIFI with an extension time of 140 s and annealing temperature of 55 °C.
Gel Electrophoresis
Hao
pOdr-10, pOdr-1, pSrb-6, pSra-10, pOsm-10, pCeh-12, pFlp-1, pStr-1
Used 0.7 g agarose in 100 mL TBE. Gel run for 55 mins at 100 V.
Microinjection
Hao Practiced injections with water.