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Revision as of 04:53, 27 October 2010 (view source) Erik (Talk | contribs) (→October 6th) ← Older edit		Revision as of 18:14, 27 October 2010 (view source) Anisha (Talk | contribs) Newer edit →
Line 12:		Line 12:
	-->J23108+LuxR (B)		-->J23108+LuxR (B)
	-->J23116+LuxR (B)		-->J23116+LuxR (B)
-		+	<center>[[Image:10-02-10.jpg]]</center>
	==October 6th==		==October 6th==
Line 37:		Line 37:
	- 5.a,b,c		- 5.a,b,c
	- 6.a,b,c		- 6.a,b,c
		+
		+	<center>[[Image:10-06-10.jpg]]</center>
	*Grown In Flasks:		*Grown In Flasks:

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Revision as of 18:14, 27 October 2010

October 5th

• Digests:

-R1062+RFP (SP)
-R0061+RFP (SP)

• Ligations:

-R1062+RFP -->J23100+LuxR (B)
           -->J23108+LuxR (B)
           -->J23116+LuxR (B)
-R0063+RFP -->J23100+LuxR (B)
           -->J23108+LuxR (B)
           -->J23116+LuxR (B)

October 6th

We selected three colonies (a,b,c) from the plates and performed a colony PCR upon each collected sample.

• Colony PCR:

1. R1062+RFP + J23100+LuxR
2. R1062+RFP + J23108+LuxR
3. R1062+RFP + J23116+LuxR
4. R0063+RFP + J23100+LuxR
5. R0063+RFP + J23108+LuxR
6. R0063+RFP + J23116+LuxR

They were then run on a gel after being PCR'd.

• Gel Order:

- 1.a,b,c
- 2.a,b,c
- 3.a,b,c
- 4.a,b,c
- 5.a,b,c
- 6.a,b,c

• Grown In Flasks:

-1a (R1062+RFP+J23100+LuxR)
-2a (R1062+RFP+J23108+LuxR)
-3a (R1062+RFP+J23116+LuxR)
-4a (R0063+RFP+J23100+LuxR)
-5a (R0063+RFP+J23108+LuxR)
-6a (R0063+RFP+J23116+LuxR)

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