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Team:WashU
From 2010.igem.org
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==Introduction== | ==Introduction== | ||
''Saccharomyces cerevisiae'' is a model unicellular eukaryotic chassis; however when compared with Escherichia coli the available synthetic biology tools are lacking. To remedy this problem the 2010 Washington University iGEM team has introduced a synthetic alternative splicing tool, as well as designed and produced new BioBricks parts to ease transformation of synthetic constructs into ''S. cerevisiae''. A mutually exclusive exon splicing system was formulated in which Sex-lethal interacts with the native splicing machinery to affect splice site choice. Two vectors have been designed to facilitate simple bacterial BioBrick manipulation and subsequent chromosomal integration into the yeast genome. A yeast positive selection marker BioBrick has been produced for the first time. Chromosomal integration with positive selection will stabilize and streamline BioBrick transformations into ''S. cerevisiae''. A synthetic splicing assembly will allow for new synthetic biology techniques such as isoform engineering of proteins or combinatorial logic. | ''Saccharomyces cerevisiae'' is a model unicellular eukaryotic chassis; however when compared with Escherichia coli the available synthetic biology tools are lacking. To remedy this problem the 2010 Washington University iGEM team has introduced a synthetic alternative splicing tool, as well as designed and produced new BioBricks parts to ease transformation of synthetic constructs into ''S. cerevisiae''. A mutually exclusive exon splicing system was formulated in which Sex-lethal interacts with the native splicing machinery to affect splice site choice. Two vectors have been designed to facilitate simple bacterial BioBrick manipulation and subsequent chromosomal integration into the yeast genome. A yeast positive selection marker BioBrick has been produced for the first time. Chromosomal integration with positive selection will stabilize and streamline BioBrick transformations into ''S. cerevisiae''. A synthetic splicing assembly will allow for new synthetic biology techniques such as isoform engineering of proteins or combinatorial logic. | ||
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==Sponsors== | ==Sponsors== | ||
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<a href="http://www.sigmaaldrich.com/sigma-aldrich/home.html"><img src="http://www.sigmaaldrich.com/etc/medialib/logos/sigma-aldrich-logo0.Par.0001.Image.gif" ></a> | <a href="http://www.sigmaaldrich.com/sigma-aldrich/home.html"><img src="http://www.sigmaaldrich.com/etc/medialib/logos/sigma-aldrich-logo0.Par.0001.Image.gif" ></a> | ||
<a href="http://engineering.wustl.edu/"><img src="http://ervin.wustl.edu/~relst/Assets/3linepos(CMYK)SM.jpg" width=172 height=150></a> | <a href="http://engineering.wustl.edu/"><img src="http://ervin.wustl.edu/~relst/Assets/3linepos(CMYK)SM.jpg" width=172 height=150></a> | ||
| - | <a href="http://www.hhmi.org/"><img src="http://depts.washington.edu/molmed/images/HHMI%20Logo.jpg" width=276 height=150></a> | + | <a href="http://www.hhmi.org/"><img src="http://depts.washington.edu/molmed/images/HHMI%20Logo.jpg" width=276 height=150></a><br /> |
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==Contact Us== | ==Contact Us== | ||
If you have any questions, advice, or are interested in joining the 2011 WashU iGEM team we would love to hear from you. The Washington University iGEM team may be reached at [mailto:WashU.iGEM@gmail.com WashU.iGEM@gmail.com] | If you have any questions, advice, or are interested in joining the 2011 WashU iGEM team we would love to hear from you. The Washington University iGEM team may be reached at [mailto:WashU.iGEM@gmail.com WashU.iGEM@gmail.com] | ||
Revision as of 04:40, 27 October 2010
Introduction
Saccharomyces cerevisiae is a model unicellular eukaryotic chassis; however when compared with Escherichia coli the available synthetic biology tools are lacking. To remedy this problem the 2010 Washington University iGEM team has introduced a synthetic alternative splicing tool, as well as designed and produced new BioBricks parts to ease transformation of synthetic constructs into S. cerevisiae. A mutually exclusive exon splicing system was formulated in which Sex-lethal interacts with the native splicing machinery to affect splice site choice. Two vectors have been designed to facilitate simple bacterial BioBrick manipulation and subsequent chromosomal integration into the yeast genome. A yeast positive selection marker BioBrick has been produced for the first time. Chromosomal integration with positive selection will stabilize and streamline BioBrick transformations into S. cerevisiae. A synthetic splicing assembly will allow for new synthetic biology techniques such as isoform engineering of proteins or combinatorial logic.
Sponsors
SM.jpg)
Contact Us
If you have any questions, advice, or are interested in joining the 2011 WashU iGEM team we would love to hear from you. The Washington University iGEM team may be reached at WashU.iGEM@gmail.com
