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Team:Purdue/MS media preparation
From 2010.igem.org
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==Procedure== | ==Procedure== | ||
| - | Use container that is twice as large as solution volume (ex. for 1 L | + | Use container that is twice as large as solution volume (ex. for 1 L of media, use a 2 L flask). Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7 using 1 N NaOH or KOH solutions. Finally, add agar. There is no need to dissolve the agar prior to autoclaving. |
==Autoclave Notes== | ==Autoclave Notes== | ||
Cover flask/container with double layer aluminum foil. Place in AutoClave and select P6-Liquid-Long Cycle. Total time for AutoClave is 60 min. | Cover flask/container with double layer aluminum foil. Place in AutoClave and select P6-Liquid-Long Cycle. Total time for AutoClave is 60 min. | ||
Revision as of 14:45, 8 July 2010
For one liter of 0.5 [http://en.wikipedia.org/wiki/Murashige_and_Skoog_medium MS]:
- 2.165 g MS salt
- 15 g sucrose (1.5%)
- 12 g agar (1.2%)
- 1 N NaOH or KOH
Procedure
Use container that is twice as large as solution volume (ex. for 1 L of media, use a 2 L flask). Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7 using 1 N NaOH or KOH solutions. Finally, add agar. There is no need to dissolve the agar prior to autoclaving.
Autoclave Notes
Cover flask/container with double layer aluminum foil. Place in AutoClave and select P6-Liquid-Long Cycle. Total time for AutoClave is 60 min.
