Team:Purdue/MS media preparation
From 2010.igem.org
(Difference between revisions)
Line 1: | Line 1: | ||
- | + | For one liter of 0.5 [http://en.wikipedia.org/wiki/Murashige_and_Skoog_medium MS]: | |
- | + | *2.165 g MS salt | |
+ | *15 g sucrose (1.5%) | ||
+ | *12 g agar (1.2%) | ||
+ | *1 N NaOH or KOH | ||
- | + | ==Procedure== | |
- | + | Use container that is twice as large as solution volume (ex. for 1 L solution one should use a 2 L flask). Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7 using 1 N NaOH or KOH solutions. Finally, add agar. There is no need to dissolve the agar prior to autoclaving. | |
- | + | ==Autoclave Notes== | |
- | + | Cover flask/container with double layer aluminum foil. Place in AutoClave and select P6-Liquid-Long Cycle. Total time for AutoClave is 60 min. | |
- | + | ||
- | + |
Revision as of 14:38, 8 July 2010
For one liter of 0.5 [http://en.wikipedia.org/wiki/Murashige_and_Skoog_medium MS]:
- 2.165 g MS salt
- 15 g sucrose (1.5%)
- 12 g agar (1.2%)
- 1 N NaOH or KOH
Procedure
Use container that is twice as large as solution volume (ex. for 1 L solution one should use a 2 L flask). Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7 using 1 N NaOH or KOH solutions. Finally, add agar. There is no need to dissolve the agar prior to autoclaving.
Autoclave Notes
Cover flask/container with double layer aluminum foil. Place in AutoClave and select P6-Liquid-Long Cycle. Total time for AutoClave is 60 min.