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Team:Cambridge/Bioluminescence/Firefly Modelling
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| - | We modelled the Luciferin cycle as described by [http://www.ncbi.nlm.nih.gov/pubmed/18949818 Marques 2009] and others. We were assisted by data from [http://www.ncbi.nlm.nih.gov/pubmed/19859663 S. Inouye (2010)] and [http://www.ncbi.nlm.nih.gov/pubmed/20655239 J.M Leitão ''et al.'' (2010)] on the inhibition of | + | We modelled the Luciferin cycle as described by [http://www.ncbi.nlm.nih.gov/pubmed/18949818 Marques 2009] and others. We were assisted by data from [http://www.ncbi.nlm.nih.gov/pubmed/19859663 S. Inouye (2010)] on the catlysis of light emission by luciferase and information [http://www.ncbi.nlm.nih.gov/pubmed/20655239 J.M Leitão ''et al.'' (2010)] on the inhibition of the enzyme. |
We used the [http://www.mathworks.com/products/simbiology/ Matlab Simbiology toolbox] to model the cycle. A diagram of the model we developed can be found below: | We used the [http://www.mathworks.com/products/simbiology/ Matlab Simbiology toolbox] to model the cycle. A diagram of the model we developed can be found below: | ||
Revision as of 02:17, 27 October 2010
We used the [http://www.mathworks.com/products/simbiology/ Matlab Simbiology toolbox] to model the cycle. A diagram of the model we developed can be found below:
Some parameters were taken from literature. However there was no clear data for others, such that we were only able to define some parameters in the light of experimental data.
While attempting to optimise our system we modelled the effect of Luciferin Regenerating Enzyme (LRE)
We modelled the system both with and without the effect of the Luciferase Regenerating Enzyme (LRE.) The 4 Figures below show a comparison the model and our data for both cases.
Our model suggested that the LRE would make light output increase faster, peak higher and decay more slowly. These were all qualities which suited us.
