Team:Osaka/Project

From 2010.igem.org

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<h3 class="CollapsiblePanelTab">Introduction</h3>  
<h3 class="CollapsiblePanelTab">Introduction</h3>  
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Desertification all over the world causes famine, drought and suffering. We aim to develop micro-machines that can stop and even reverse desertification by recovering vegetation in these areas. We envison a <i>Continuous Greening Cycle</i> in which engineered microorganisms decompose plant fibers into nutrients through the action of cellulolytic enzymes. They then produce water-holding polymers such as poly(gamma-glutamic) acid that retain water in the soil to help plants grow. When the plants die they will be decomposed to start the cycle anew. In addition to aiming for the continuous and self-expanding greening of desert areas, we hope to contribute to iGEM by developing useful BioBricks!</p>
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Desertification all over the world causes famine, drought and suffering. We aim to develop micro-machines that can stop and even reverse desertification by recovering vegetation in these areas.  
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</p>
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<h3><a href="Project_cellulase">Cellulase</a></h3>
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<h3>Objectives</h3>
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<p>We had 3 objectives in mind when planning this project:
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<ol>
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<li>To address environmental issue of desertification</li>
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<li>To feasibility of designing and implementing a cyclic biological system</li>
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<li>To contribute to iGEM and Synthetic Biology by developing and characterizing new parts</li>
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</p>
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<h3><a href="Project_pga">Gamma polyglutamic acid</a></h3>
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<h3>The Cycle</h3>
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<p>We envisioned a <b>Continuous Greening Cycle</b> in which engineered microorganisms decompose plant fibers into nutrients through the action of cellulolytic enzymes. They then produce water-holding polymers such as poly(gamma-glutamic) acid that retain water in the soil to help plants grow. When the plants die they will be decomposed to start the cycle anew.</p>
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<h3><a href="Parts">Parts</a></h3>
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<h3>Cellulose degradation</h3>
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<p><a href="Project_cellulase">Cellulase</a></p>
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<h3><a href="Tests">Tests</a></h3>
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<h3>Polyglutamic acid (PGA) synthesis</h3>
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<a href="Project_pga">Gamma polyglutamic acid</a>
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<h3><a href="Modeling">Modeling</a></h3>
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<h3></h3>
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<p>Construction of the parts for cellulose degradation and PGA synthesis outlined above formed the majority of our wet lab work. We produced a collection of new BioBricks by PCR from existing plasmids or genome DNA, and also made some constructs to test the new parts. For more info, please see <a href="Parts">Parts</a>.</p>
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<h3>Results Summary</h3>
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<h3>Tests</h3>
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<p>We ran several tests to confirm the working of our parts, as well as characterize them quantitatively. See the <a href="Tests">Tests</a> page for more info.</p>
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<h3>Modeling</h3>
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<p>We also attempted to construct a model and simulate it using software in order to determine the feasibility of the cycle as well as identify important parameters involved. See the <a href="Modeling">Modeling</a> page for more info.</p>
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<h3>Results Summary</h3>
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Revision as of 04:06, 27 October 2010

Project: The Continuous Greening Cycle

Introduction

Desertification all over the world causes famine, drought and suffering. We aim to develop micro-machines that can stop and even reverse desertification by recovering vegetation in these areas.

Objectives

We had 3 objectives in mind when planning this project:

  1. To address environmental issue of desertification
  2. To feasibility of designing and implementing a cyclic biological system
  3. To contribute to iGEM and Synthetic Biology by developing and characterizing new parts

    4. The Cycle

    We envisioned a Continuous Greening Cycle in which engineered microorganisms decompose plant fibers into nutrients through the action of cellulolytic enzymes. They then produce water-holding polymers such as poly(gamma-glutamic) acid that retain water in the soil to help plants grow. When the plants die they will be decomposed to start the cycle anew.

    Cellulose degradation

    Cellulase

    Polyglutamic acid (PGA) synthesis

    Gamma polyglutamic acid

    Construction of the parts for cellulose degradation and PGA synthesis outlined above formed the majority of our wet lab work. We produced a collection of new BioBricks by PCR from existing plasmids or genome DNA, and also made some constructs to test the new parts. For more info, please see Parts.

    Tests

    We ran several tests to confirm the working of our parts, as well as characterize them quantitatively. See the Tests page for more info.

    Modeling

    We also attempted to construct a model and simulate it using software in order to determine the feasibility of the cycle as well as identify important parameters involved. See the Modeling page for more info.

    Results Summary

    (delete??)

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