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Team:Michigan/Quorum Sensing
From 2010.igem.org
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| + | =Quorum Sensing Team Lab Notebooks= | ||
==7/7/10== | ==7/7/10== | ||
''Jennifer and Alex - in Lin Lab'' | ''Jennifer and Alex - in Lin Lab'' | ||
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Inoculated ''E. coli'' DH5α from frozen stock into 12 mL LB broth | Inoculated ''E. coli'' DH5α from frozen stock into 12 mL LB broth | ||
*put in 30°C, 200 rpm shaking, overnight | *put in 30°C, 200 rpm shaking, overnight | ||
| + | |||
| + | == 7/8/10 == | ||
| + | ''Alex, Jennifer and Eric - in ERB'' | ||
| + | Made LB agar plates w/ ampicillin | ||
| + | -10 g LB broth | ||
| + | -7.5 g agar | ||
| + | -500 mL DI water | ||
| + | *autoclaved mixture at 121°C for 30 min (sterilize time), following Mike Nelson's protocol | ||
| + | ---- | ||
| + | Created protocols for getting deionized water and using spectrophotometer | ||
| + | *uploaded to protocols section | ||
| + | ---- | ||
Revision as of 19:34, 8 July 2010
Quorum Sensing Team Lab Notebooks
7/7/10
Jennifer and Alex - in Lin Lab
Made CaCl2 stock solution: 0.1M - 50mL
- molar mass = 111 g/mol
- (.1 mol/L)(.05 L)(111 g/mol) = 555mg
- dissolved 555 mg CaCl2 in 50mL DIwater
- vacuum filtered into 50mL tube
Made ampicillin stock solution:
-1 g ampicillin -5mL DIwater -ethanol
- filtered by syringe into 15mL tube
- put into ten 1mL alliquots in 1.5mL tubes
- stored in -20°C in ERB lab
Inoculated E. coli DH5α from frozen stock into 12 mL LB broth
- put in 30°C, 200 rpm shaking, overnight
7/8/10
Alex, Jennifer and Eric - in ERB Made LB agar plates w/ ampicillin
-10 g LB broth -7.5 g agar -500 mL DI water
- autoclaved mixture at 121°C for 30 min (sterilize time), following Mike Nelson's protocol
Created protocols for getting deionized water and using spectrophotometer
- uploaded to protocols section
