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Team:Michigan/Media Recipes
From 2010.igem.org
(Difference between revisions)
| Line 46: | Line 46: | ||
*2.5 mL of ethanol | *2.5 mL of ethanol | ||
''filter sterilize and make 1 mL aliquots to store in the -20°C freezer'' | ''filter sterilize and make 1 mL aliquots to store in the -20°C freezer'' | ||
| + | |||
| + | '''IPTG stock solution (1 M)''' | ||
| + | *0.25 g of tetracycline | ||
| + | *1049 uL of DI water | ||
| + | ''filter sterilize with .22 um filter'' | ||
Revision as of 16:27, 27 July 2010
Media Recipes
LB media
- 10 g LB broth
- 500 mL DI water
autoclave to sterilize
LB media with agar
- 10 g LB broth
- 7.5 g agar
- 500 mL DI water
autoclave to sterilize
add antibiotics after autoclaving when warm to touch (about 50°C)
pour plates when warm to the touch (about 50°C)
0.1 M CaCl2 solution
- 0.555 g of CaCl2
- 50 mL of DI water
filter sterilize
Ampicillin Antibiotic Stock Solution (100 mg/mL)
- 1 g of ampicillin
- 5 mL of DI water
- 5 mL of ethanol
filter sterilize and make 1 mL aliquotes to store in the -20°C freezer
Kanamycin Antibiotic Stock Solution (50 mg/mL)
- 250 mg of kanamycin
- 5 mL of DI water
filter sterilize and make 1 mL aliquots to store in the -20°C freezer
Tetracycline Antibiotic Stock Solution (50 mg/mL)
- 250 mg of tetracycline
- 2.5 mL of DI water
- 2.5 mL of ethanol
filter sterilize and make 1 mL aliquots to store in the -20°C freezer
IPTG stock solution (1 M)
- 0.25 g of tetracycline
- 1049 uL of DI water
filter sterilize with .22 um filter
