"
Team:Wisconsin-Madison/results
From 2010.igem.org
| Line 68: | Line 68: | ||
====Conclusion==== | ====Conclusion==== | ||
| + | |||
| + | <br> | ||
===Cell Survivability in Acidic Conditions=== | ===Cell Survivability in Acidic Conditions=== | ||
====Background==== | ====Background==== | ||
| Line 75: | Line 77: | ||
====Results==== | ====Results==== | ||
[[Image:Cell Survivability Testing Graphs.jpg|center]] | [[Image:Cell Survivability Testing Graphs.jpg|center]] | ||
| - | + | ||
====Conclusion==== | ====Conclusion==== | ||
| - | + | <br> | |
==Timed Lysis: Inducible/Repressible Promoter== | ==Timed Lysis: Inducible/Repressible Promoter== | ||
{| border="1" cellpadding="5" cellspacing="0" align="center" | {| border="1" cellpadding="5" cellspacing="0" align="center" | ||
Revision as of 17:56, 26 October 2010
Encapsulation
| Part Number | Function | Expression Type | Zip File |
| <partinfo>BBa_k318500</partinfo> | Produces Trascription Factor RcsB | Inducible - IPTG | 500 |
| <partinfo>BBa_k318501</partinfo> | Produces Trascription Factor RcsA | Inducible - IPTG | 501 |
| <partinfo>BBa_k318502</partinfo> | Produces Trascription Factor RcsA & RcsB | Inducible - IPTG | 502 |
| <partinfo>BBa_k200021</partinfo> | Empty Vector/Contol | Inducible - IPTG | NA |
Colonic Acid Quantification
Background
Colonic Acid is a polysaccharide containing a repeat unit with D-glucose, L-fucose, D-galactose, and D-glucuronate. Biological extracts often contain compounds, which under heating with H2SO4 yield brown products absorbing between 396 nm and 427 nm. Colonic acid can be estimated by measuring L-fucose content.
Download procedure here or continue to experimental protocol page
Results
Conclusion
Cell Survivability in Acidic Conditions
Background
Download procedure here or continue to experimental protocol page
Results
Conclusion
Timed Lysis: Inducible/Repressible Promoter
| Part Number | Function | Induction | Zip File |
| <partinfo>BBa_k318513</partinfo> | Produces RFP | stationary phase | 513 |
Background
gadAp: A pH Sensitive Promoter Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part
pH Induction Results
Download procedure here or continue to experimental protocol page
Stationary Phase Induction Results
Download procedure here or continue to experimental protocol page
Conclusion
