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Team:Wisconsin-Madison/results
From 2010.igem.org
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====Results==== | ====Results==== | ||
[[Image:Cell Survivability Testing Graphs.jpg|center]] | [[Image:Cell Survivability Testing Graphs.jpg|center]] | ||
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==Timed Lysis: Inducible/Repressible Promoter== | ==Timed Lysis: Inducible/Repressible Promoter== | ||
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| - | ===Background== | + | ===Background=== |
'''gadAp: A pH Sensitive Promoter''' | '''gadAp: A pH Sensitive Promoter''' | ||
Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part | Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part | ||
Revision as of 17:52, 26 October 2010
Encapsulation
| Part Number | Function | Expression Type | Zip File |
| <partinfo>BBa_k318500</partinfo> | Produces Trascription Factor RcsB | Inducible - IPTG | 500 |
| <partinfo>BBa_k318501</partinfo> | Produces Trascription Factor RcsA | Inducible - IPTG | 501 |
| <partinfo>BBa_k318502</partinfo> | Produces Trascription Factor RcsA & RcsB | Inducible - IPTG | 502 |
| <partinfo>BBa_k200021</partinfo> | Empty Vector/Contol | Inducible - IPTG | NA |
Colonic Acid Quantification
Background
Colonic Acid is a polysaccharide containing a repeat unit with D-glucose, L-fucose, D-galactose, and D-glucuronate. Biological extracts often contain compounds, which under heating with H2SO4 yield brown products absorbing between 396 nm and 427 nm. Colonic acid can be estimated by measuring L-fucose content.
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Results
Cell Survivability in Acidic Conditions
Background
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Results
Timed Lysis: Inducible/Repressible Promoter
| Part Number | Function | Induction | Zip File |
| <partinfo>BBa_k318513</partinfo> | Produces RFP | stationary phase | 513 |
Background
gadAp: A pH Sensitive Promoter Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part
pH Induction Results
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Stationary Phase Induction Results
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