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Team:Wisconsin-Madison/results
From 2010.igem.org
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Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part | Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part | ||
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| + | ===pH Induction=== | ||
| + | ===Stationary Phase Induction=== | ||
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Revision as of 17:44, 26 October 2010
Encapsulation
Colonic Acid Quantification
Encapsulation: Cell Survivability in Acidic Conditions
Timed Lysis: Inducible/Repressible Promoter
gadAp: A pH Sensitive Promoter
Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part
pH Induction
Stationary Phase Induction
