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	=== Making the Inducible T7 Expression Vector ===		=== Making the Inducible T7 Expression Vector ===
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		+	The aim for this section is to create a an inducible expression backbone. this will be achieved by altering the backbone biobrick '''BBa_J04500''', by removing the -35 and -10 elements (promoter) and inserting the bacteriophage T7 promoter. the strategy used to will be Quikchange XL mutagenesis to alter the promoter elements into restriction sites:
		+	* -35 --> AflII
		+	* -10 --> BamH1
		+
		+	These restriction sites are NOT found in the BBa_J04500 part or vector (pSB1A2), so there will be no cross cutting.
	== Results ==		== Results ==

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Revision as of 21:14, 6 July 2010

Overall project

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Project Details

Creation of the Taq Polymerase Part

Making the Inducible T7 Expression Vector

The aim for this section is to create a an inducible expression backbone. this will be achieved by altering the backbone biobrick BBa_J04500, by removing the -35 and -10 elements (promoter) and inserting the bacteriophage T7 promoter. the strategy used to will be Quikchange XL mutagenesis to alter the promoter elements into restriction sites:

• -35 --> AflII
• -10 --> BamH1

These restriction sites are NOT found in the BBa_J04500 part or vector (pSB1A2), so there will be no cross cutting.

Results