Team:Cambridge/Tools/Lighting
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A tree in this position would be able to photosynthesise during the day, building up reserves of energy. We then imagined it emitting light by night, using the bacterial luciferase system, under the control of the inherent circadian clock based gene regulation systems. | A tree in this position would be able to photosynthesise during the day, building up reserves of energy. We then imagined it emitting light by night, using the bacterial luciferase system, under the control of the inherent circadian clock based gene regulation systems. | ||
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{{:Team:Cambridge/Templates/RightImage|image=Jungle_book.jpg|caption=''Ben proving it is possible to read by bioluminescent light''}} | {{:Team:Cambridge/Templates/RightImage|image=Jungle_book.jpg|caption=''Ben proving it is possible to read by bioluminescent light''}} | ||
We wanted to provide some proof of concept of these ideas. We built the [http://www.youtube.com/watch?v=tUFscEVK5Ks bacterial bubble lamp] to investigate this, and also read the Jungle Book using a flask containing E. coli expressing our bacterial luciferase. | We wanted to provide some proof of concept of these ideas. We built the [http://www.youtube.com/watch?v=tUFscEVK5Ks bacterial bubble lamp] to investigate this, and also read the Jungle Book using a flask containing E. coli expressing our bacterial luciferase. | ||
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+ | We had thought about bacteria being used for emergency lighting, and our advisor Fernan printed a fire exit sign, which was placed over a plate containing one of our strains of E. coli. In a real system we would imagine using anhydrobiosis to create bacteria 'in hibernation' which could be activated when needed. | ||
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+ | <img src="https://static.igem.org/mediawiki/2010/b/ba/Fire-exit-horiz.jpg"></div> | ||
+ | <div style="margin-top:5px; font-size:12px; color:#6e6e6e; line-height:15px; text-align:center">Our <a href="https://2010.igem.org/Team:Cambridge/Bioluminescence/G28"> LuxBrick</a> with an overlay to give a meaningful message</div> | ||
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=Modelling= | =Modelling= |
Revision as of 17:34, 26 October 2010
Bioluminescent street lamps
In order to provide any solution to the problem, a biological solution must tap into a currently unused energy resource. For this reason we decided to consider the use of bioluminescent trees to replace conventional street lamps.
A tree in this position would be able to photosynthesise during the day, building up reserves of energy. We then imagined it emitting light by night, using the bacterial luciferase system, under the control of the inherent circadian clock based gene regulation systems.
Putting it into practice
We wanted to provide some proof of concept of these ideas. We built the [http://www.youtube.com/watch?v=tUFscEVK5Ks bacterial bubble lamp] to investigate this, and also read the Jungle Book using a flask containing E. coli expressing our bacterial luciferase.
We had thought about bacteria being used for emergency lighting, and our advisor Fernan printed a fire exit sign, which was placed over a plate containing one of our strains of E. coli. In a real system we would imagine using anhydrobiosis to create bacteria 'in hibernation' which could be activated when needed.
Modelling
To show that it may one day indeed be possible to have bioluminescent trees replacing street lamps we considered how efficicient the plants would have to be in order to match a low-intensity street lamp.
Figure 1 shows the radiation spectrum recieved from the sun at sea level. In total this gives about 1,321 – 1,413 W/m2 of radiant energy for regions in North America. (Data supplied by American Society for Testing and Materials ([http://www.wmo.int/pages/prog/www/IMOP/publications/CIMO-Guide/CIMO%20Guide%207th%20Edition,%202008/Part%20I/Chapter%208.pdf. ASTM]) Terrestrial Reference Spectra, a common standard used in photovoltaics).
This is a lot of energy, but of course most of it is not accessible to plants. They can only absorb in the visible region (corresponding to roughy 45% of total solar energy), radiation known as PAR (photosynthetically active radiation). In addition, there are other constraints, such as reflectivity of leaves and the absorption spectrum of chlorophyll. The net result is that in general plants are only able to take between 3 and 6% of total solar radiation, corresponding to roughly 60 W/m2 (Figures from Hall, D.O. and House, J.I., Biomass and Bioenergy, 6,11-30 (1994).)
That's what plants have available to them, we then considered what they need to be putting out in order to function as street lights.
'''Light Source''' | '''Wattage''' | '''Output(lumens)''' |
Incandescent | 25-150 | 210-2700 |
Fluorescent | 18-95 | 1000-7500 |
Metal Halide | 50-400 | 1900-30000 |
High-Pressure Sodium | 50-400 | 3600-46000 |
Low-Pressure Sodium | 18-180 | 1800-33000 |
1,413 – 1,321W/m2 "Chapter 8 – Measurement of sunshine duration" (PDF). CIMO Guide. World Meteorological Organization. http://www.wmo.int/pages/prog/www/IMOP/publications/CIMO-Guide/CIMO%20Guide%207th%20Edition,%202008/Part%20I/Chapter%208.pdf. Retrieved 2008-12-01.