Team:Stockholm/23 June 2010
From 2010.igem.org
(Difference between revisions)
(→Andreas) |
|||
Line 3: | Line 3: | ||
=Andreas= | =Andreas= | ||
- | === | + | ===Testing Top10 competent cells and antibiotic agar plates=== |
+ | |||
+ | ''Results from 22/6'' | ||
+ | |||
+ | {| border="1" cellspacing="0" cellpadding="2" | ||
+ | |'''Transformed plasmid''' | ||
+ | |'''Plate''' | ||
+ | |'''Growth''' | ||
+ | |- | ||
+ | |pEX | ||
+ | |100 ug/ml Amp | ||
+ | |Yes | ||
+ | |- | ||
+ | |None | ||
+ | |100 ug/ml Amp | ||
+ | |No | ||
+ | |- | ||
+ | |pEX | ||
+ | |25 ug/ml Cm | ||
+ | |No | ||
+ | |- | ||
+ | |None | ||
+ | |25 ug/ml Cm | ||
+ | |No | ||
+ | |} | ||
+ | |||
+ | Growth on Amp plate indicated transformation competence. No growth on other plates indicated good antibiotic selection. | ||
+ | |||
+ | '' '''Note:''' The transformed pEX vector was thought to carry a Cm resistance cassette insert. This was, however, discovered to be wrong.'' | ||
=Hassan= | =Hassan= |
Revision as of 09:16, 6 July 2010
Andreas
Testing Top10 competent cells and antibiotic agar plates
Results from 22/6
Transformed plasmid | Plate | Growth |
pEX | 100 ug/ml Amp | Yes |
None | 100 ug/ml Amp | No |
pEX | 25 ug/ml Cm | No |
None | 25 ug/ml Cm | No |
Growth on Amp plate indicated transformation competence. No growth on other plates indicated good antibiotic selection.
Note: The transformed pEX vector was thought to carry a Cm resistance cassette insert. This was, however, discovered to be wrong.
Hassan
Started to use softwares for making 3D animations and/or movies, started by making protein A and IgG Protease.
softwares used:
- Molecular Maya
- Chimera