Team:MIT mammalian Bone
From 2010.igem.org
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<b>In Vitro Osteoblast Differentiation</b> | <b>In Vitro Osteoblast Differentiation</b> | ||
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- | Our first task was to see if we could induce osteogenic differentiation in our stem cell lines. We obtained a stock of human recombinant BMP2 protein and added it to the supernatant of stem cell cultures at 100ng/ml and 300ng/ml, based on a review of concentrations used in the literature. | + | Our first task was to see if we could induce osteogenic differentiation in our stem cell lines. We obtained a stock of human recombinant BMP2 protein and added it to the supernatant of stem cell cultures at 100ng/ml and 300ng/ml, based on a review of concentrations used in the literature. We tested the response to both constitutive (all 5 days) and transient (induction stopped on day 2) BMP2 signaling. Micrographs were taken on day 5 to assay for changes morphology. |
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<a href="https://static.igem.org/mediawiki/2010/e/ed/Bone_ALP_Assay_7_6_2010-2.jpg" target="_blank"> <img width=600px src="https://static.igem.org/mediawiki/2010/e/ed/Bone_ALP_Assay_7_6_2010-2.jpg"> </a> | <a href="https://static.igem.org/mediawiki/2010/e/ed/Bone_ALP_Assay_7_6_2010-2.jpg" target="_blank"> <img width=600px src="https://static.igem.org/mediawiki/2010/e/ed/Bone_ALP_Assay_7_6_2010-2.jpg"> </a> | ||
<b> References </b> | <b> References </b> |
Revision as of 13:29, 26 October 2010