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	==Protocols==		==Protocols==
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		+	'''Using Lab Equipment'''
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		+	[[Media:6-28-2010_Protocol_for_making_cultures_from_a_-80C_freezer_stock.pdf|Enigeering Research Building Autoclave]]
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	'''Cell Culture'''		'''Cell Culture'''

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Revision as of 20:32, 6 July 2010

General Outline for Biobrick Manipulations

Protocols

Using Lab Equipment

Enigeering Research Building Autoclave

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

DNA Manipulation

Transformation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation

Miniprep

DNA Digest

Running a Gel

DNA Purification

Ligation

• Gingo Bioworks Protocol
• Jeremy Minty Protocol