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		+	==General Outline for Biobrick Manipulations==
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Revision as of 22:12, 5 July 2010

General Outline for Biobrick Manipulations

Protocols

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

DNA Manipulation

Transformation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation

Miniprep

DNA Digest

Running a Gel

DNA Purification

Ligation

• Gingo Bioworks Protocol
• Jeremy Minty Protocol