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From 2010.igem.org

Revision as of 21:12, 5 July 2010 (view source) Annlesn (Talk | contribs) ← Older edit		Revision as of 21:13, 5 July 2010 (view source) Annlesn (Talk | contribs) Newer edit →
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	DNA Manipulation		DNA Manipulation
-	[[Media:6-27-2010_P._putida_antibiotic_resistance_protocol.pdf|Transformation]]	+	[[Media:General_Transformation_Protocol.pdf|Transformation]]
	*Getting parts from the 360 well registry plates		*Getting parts from the 360 well registry plates
	*Competent cell preparation		*Competent cell preparation
	*Electroporation		*Electroporation

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Revision as of 21:13, 5 July 2010

Protocols

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

DNA Manipulation

Transformation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation