Team:Stockholm/17 October 2010
From 2010.igem.org
(Difference between revisions)
(→Gel) |
|||
Line 43: | Line 43: | ||
==== Gel ==== | ==== Gel ==== | ||
- | [[Image: | + | [[Image:2010-10-17_pEX.SOD_operon.jpg|250px|thumb|left|]] |
{| | {| | ||
! well | ! well | ||
Line 89: | Line 89: | ||
| pC.Sh_yC 4 | | pC.Sh_yC 4 | ||
|} | |} | ||
- | |||
- | |||
- | |||
==== new colony PCR ==== | ==== new colony PCR ==== |
Revision as of 14:51, 26 October 2010
Contents |
Mimmi
clone non-CPP operon
colony PCR
mix | x12 | primers | samples | |||
---|---|---|---|---|---|---|
mastermix | 24.5 | pEX_VF | pEX.SOD.RBS.yCCS 1-4 | |||
DNA | 0.5 | pEX_VR | pEX.SOD.his.RBS.yCCS 1-4 | |||
tot | 25µl | pEX.his.SOD.RBS.yCCS 1-4 | ||||
positive control (pSB1C3.LMWP.SOD.his) |
Gel
well | sample | well | sample |
---|---|---|---|
1 | ladder | 12 | ladder |
2 | pEX.S_yC 1 | 13 | pEX.hS_yC 1 |
3 | pEX.S_yC 2 | 14 | pEX.hS_yC 2 |
4 | pEX.S_yC 3 | 15 | pEX.hS_yC 3 |
5 | pEX.S_yC 4 | 16 | pEX.hS_yC 4 |
6 | pEX.Sh_yC 1 | ||
7 | pC.Sh_yC 2 | ||
8 | pC.Sh_yC 3 | ||
9 | pC.Sh_yC 4 |
new colony PCR
mix | x7 | primers | samples | |||
---|---|---|---|---|---|---|
mastermix | 24.5 | pEX_VF | pEX.SOD.his.RBS.yCCS 1-7 | |||
DNA | 0.5 | pEX_VR | ||||
tot | 25µl |
ON cultures
- pEX.SOD.RBS.yCCS 4
- pEX.his.SOD.RBS.yCCS 3
- pEX.SOD
- pEX.yCCS
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |