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Team:Stockholm/21 June 2010
From 2010.igem.org
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== Preparation of competent Top10 Escherichia coli cells == | == Preparation of competent Top10 Escherichia coli cells == | ||
* Set a 5 ml LB ON culture; 37°C, 250 rpm. | * Set a 5 ml LB ON culture; 37°C, 250 rpm. | ||
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| + | =Johan= | ||
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| + | Overnight culture of pEX | ||
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| + | 3 falcon tubes (50 ml), 10 ml LB in each. | ||
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| + | 24/12=2 | ||
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| + | 2*30=60µl Amp | ||
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| + | with tip from pEX glycerol stock | ||
{{Stockholm/Footer}} | {{Stockholm/Footer}} | ||
Latest revision as of 18:04, 27 October 2010
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Contents |
Andreas
Preparation LB agar plates with antibiotics
- 100 ug/ml Amp (20 plates)
- 25 ug/ml Cm (20 plates)
Preparation of competent Top10 Escherichia coli cells
- Set a 5 ml LB ON culture; 37°C, 250 rpm.
Johan
Overnight culture of pEX
3 falcon tubes (50 ml), 10 ml LB in each.
24/12=2
2*30=60µl Amp
with tip from pEX glycerol stock
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