Team:UNIPV-Pavia/Calendar/June/settimana4

From 2010.igem.org

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Revision as of 08:11, 5 July 2010

JUNE: WEEK 4

June, 21st

Planning of the activity of the week. Freezer cleaning: for each ligation, we choosed the correct clone and stored it in the iGEM 2010 ligations box. All these cloned were gel screened and sequenced and re correct! The colonies we choosed are:

colony choosed	ligation name
I0-2	I0
I1-2	I1
I2-1	I2
I3-1	I3
I4-2	I4
I5-1	I5
I6-2	I6

Other colonies resulted psitives at the screening but were NOT sequenced and are stored in the iGEM 2010 cemetery box. These clones are: I1-1, I2-2, I4-1 and I6-3.

June, 22nd

Inoculum of I6, <partinfo>BBa_J23118</partinfo>, <partinfo>BBa_J23110</partinfo>, <partinfo>BBa_J23114</partinfo>, <partinfo>BBa_J23116</partinfo> from glycerol stock in 5ml LB+Amp. Cultures were grown ON at 37°C 220rpm.

June, 23rd

June, 24th

June, 25th

Screening of I7, I8, I9, I10

June

week 2

week 3

week 4

@@ Line 34: / Line 34: @@
 ==June, 22nd==
-[[Image:Unipv_digestion_J231xx_I6.jpg|300px|thumb|center|J231xx and I6 digestion]]
+Inoculum of I6, <partinfo>BBa_J23118</partinfo>, <partinfo>BBa_J23110</partinfo>, <partinfo>BBa_J23114</partinfo>, <partinfo>BBa_J23116</partinfo> from glycerol stock in 5ml LB+Amp. Cultures were grown ON at 37°C 220rpm.
 ==June, 23rd==