Revision as of 04:35, 26 October 2010

Week #5

Let's picked up colonys our transformant cells and prepare all to do miniprep.

For this we used 30ml de LB liquid agar medium per falcon tube at 80μ/ml

Cloramphenicol concentration we left overnight culture to 37º

With a overnight inoculum we did a miniprep.

The miniprep was done with a Quiagen Kit.

Then we going to do a duble digest with EcoRI and PstI to check out our ligations.

We run a gel to confirm our ligations as follow

We prepare all to repeat ligation; PCR2 with Psb1C3 and PCR4 with Psb1C3

Revision as of 04:28, 26 October 2010 (view source) Jesús (Talk \| contribs) ← Older edit		Revision as of 04:35, 26 October 2010 (view source) Jesús (Talk \| contribs) Newer edit →
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-	'''We prepare all ~~for~~ repeat ligation PCR2 with Psb1C3 and PCR4 with Psb1C3'''	+	'''We prepare all to repeat ligation; PCR2 with Psb1C3 and PCR4 with Psb1C3'''