"
Team:Nevada/Agrobacterium Transformations
From 2010.igem.org
(Difference between revisions)
(→Agrobacterium Transformations) |
|||
| Line 38: | Line 38: | ||
<br> | <br> | ||
<br> | <br> | ||
| - | <html><a href="https://static.igem.org/mediawiki/2010/d/d0/Agro_theory.png"><img src="https://static.igem.org/mediawiki/2010/d/d0/Agro_theory.png" class="shadow" style="float:left;width: | + | <html><a href="https://static.igem.org/mediawiki/2010/d/d0/Agro_theory.png"><img src="https://static.igem.org/mediawiki/2010/d/d0/Agro_theory.png" class="shadow" style="float:left;width:900px;margin:10px"></a> |
</html> | </html> | ||
Revision as of 02:44, 26 October 2010
Agrobacterium Transformations
Agrobacterium tumefaciens Mediated Transformation of Plant Cells
Agrobacterium tumefaciens
- Gram (-) soil bacterium
- Causative agent in crown gall disease
- Disease is the result of interkingdom transfer of DNA (i.e. bacteria --> plant)
Molecular Progression of Agrobacterium Infection
1. chemical perception of host
2. induction of virulence genes
3. formation of infection apparatus
4. preparation of T-DNA
5. export T-DNA to plant cell
6. import T-DNA to plant nucleus
7. integration of T-DNA into host genome
8. expression of T-DNA
