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Team:Berkeley/Results
From 2010.igem.org
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Results of Delivery of GFP payload with Payload Delivery Device | Results of Delivery of GFP payload with Payload Delivery Device | ||
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In general to assay payload delivery, we first | In general to assay payload delivery, we first | ||
# Feed the payload bacteria to the choanos and induce self-lysis, not necessarily at the same time. | # Feed the payload bacteria to the choanos and induce self-lysis, not necessarily at the same time. | ||
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SInce the payload we used in our assays was GFP, we assayed for delivery events using fluorescent microscopy. | SInce the payload we used in our assays was GFP, we assayed for delivery events using fluorescent microscopy. | ||
| - | + | '''Challenges''' | |
The major challenge we faced with assaying for payload delivery was determining the timing of when to induce self-lysis and when to look at the choanos under the microscope.[[Image:timeline.png|700px]] | The major challenge we faced with assaying for payload delivery was determining the timing of when to induce self-lysis and when to look at the choanos under the microscope.[[Image:timeline.png|700px]] | ||
Revision as of 02:09, 26 October 2010
- Home
- Project
- Parts
- Self-Lysis
- Vesicle-Buster
- Payload
- [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=Berkeley Parts Submitted]
- Results
- Judging
- Clotho
- Human Practices
- Team Resources
- Who We Are
- Notebooks:
- [http://www.openwetware.org/wiki/Berk2010-Daniela Daniela's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Christoph Christoph's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Amy Amy's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Tahoura Tahoura's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Conor Conor's Notebook]
Results of Delivery of GFP payload with Payload Delivery Device
Experimental Set Up In general to assay payload delivery, we first
- Feed the payload bacteria to the choanos and induce self-lysis, not necessarily at the same time.
- Next, we assay for successful delivery events.
SInce the payload we used in our assays was GFP, we assayed for delivery events using fluorescent microscopy.
Challenges
The major challenge we faced with assaying for payload delivery was determining the timing of when to induce self-lysis and when to look at the choanos under the microscope.
