Team:Michigan/Project
From 2010.igem.org
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The goals of the quorum sensing team are two-fold: first to characterize the response of ''E. coli'' to the ''C. vulgaris'' AI-2 mimic (which may be actual AI-2), and second to engineer ''E. coli'' to flocculate in response to the presence of ''C. vulgaris''. The first task will be accomplished by transforming a LuxS-mutant strain of ''E. coli'' (cannot produce its own AI-2) with an AI-2 reporter biobrick. We will then harvest supernatant from ''C. vulgaris'', which should contain AI-2 or its mimic, and apply it to the reporter strain to test its response. The second task will be accomplished by ligating a gene that causes over-expression of pili (characterized by the pili team) to the Lsr promoter, which is derepressed in response to AI-2. By transforming this part into LuxS-mutant ''E. coli'', we hope to create strain that will stick to algae and will flocculate only in the presence of ''C. vulgaris''. | The goals of the quorum sensing team are two-fold: first to characterize the response of ''E. coli'' to the ''C. vulgaris'' AI-2 mimic (which may be actual AI-2), and second to engineer ''E. coli'' to flocculate in response to the presence of ''C. vulgaris''. The first task will be accomplished by transforming a LuxS-mutant strain of ''E. coli'' (cannot produce its own AI-2) with an AI-2 reporter biobrick. We will then harvest supernatant from ''C. vulgaris'', which should contain AI-2 or its mimic, and apply it to the reporter strain to test its response. The second task will be accomplished by ligating a gene that causes over-expression of pili (characterized by the pili team) to the Lsr promoter, which is derepressed in response to AI-2. By transforming this part into LuxS-mutant ''E. coli'', we hope to create strain that will stick to algae and will flocculate only in the presence of ''C. vulgaris''. | ||
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[[Image:QS_circuit.jpg|(Li, et. al.)]] | [[Image:QS_circuit.jpg|(Li, et. al.)]] | ||
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+ | === Results to Date === | ||
[[Media:QS_culture_test_8-5-10.pdf]] | [[Media:QS_culture_test_8-5-10.pdf]] |
Revision as of 23:48, 25 October 2010