Team:Sheffield/Chimeric protein system
From 2010.igem.org
(Difference between revisions)
Line 1: | Line 1: | ||
{{Template:Team:Sheffield/Navigation2}} | {{Template:Team:Sheffield/Navigation2}} | ||
- | |||
- | |||
- | |||
<html xmlns="http://www.w3.org/1999/xhtml"> | <html xmlns="http://www.w3.org/1999/xhtml"> | ||
Line 13: | Line 10: | ||
<table width="850" border="0" align="center" cellpadding="0" cellspacing="0"> | <table width="850" border="0" align="center" cellpadding="0" cellspacing="0"> | ||
<tr> | <tr> | ||
- | <td><div align="left"><img src="https://static.igem.org/mediawiki/2010/5/51/Modellinglogo.jpg" alt="modelling logo" width="400" height="100" /></div></td> | + | <td width="850"><div align="left"><img src="https://static.igem.org/mediawiki/2010/5/51/Modellinglogo.jpg" alt="modelling logo" width="400" height="100" /></div></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><p> </p> | + | <td width="850"><p> </p> |
<p><a href="https://2010.igem.org/Team:Sheffield/Chimeric_protein_system" target="_self">Chimeric protein system</a> | <a href="https://2010.igem.org/Team:Sheffield/cholera_protein_system" target="_self">Cholera protein system</a> | <a href="https://2010.igem.org/Team:Sheffield/Graphical_User_Interface" target="_self">Graphical user interface</a></p> </td> | <p><a href="https://2010.igem.org/Team:Sheffield/Chimeric_protein_system" target="_self">Chimeric protein system</a> | <a href="https://2010.igem.org/Team:Sheffield/cholera_protein_system" target="_self">Cholera protein system</a> | <a href="https://2010.igem.org/Team:Sheffield/Graphical_User_Interface" target="_self">Graphical user interface</a></p> </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><div> | + | <td width="850"><div> |
<div id="ftn1"> | <div id="ftn1"> | ||
<p> </p> | <p> </p> | ||
Line 27: | Line 24: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><img src="https://static.igem.org/mediawiki/2010/4/4e/Chimericprotein.jpg" alt="GUI Logo" width="400" height="100" /></td> | + | <td width="850"><img src="https://static.igem.org/mediawiki/2010/4/4e/Chimericprotein.jpg" alt="GUI Logo" width="400" height="100" /></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><p><strong>Explanation of the system</strong></p> | + | <td width="850"><p><strong>Explanation of the system</strong></p> |
<p>The diagram below explains the process of our cholera detection biosensor with the chimeric protein system.</p> | <p>The diagram below explains the process of our cholera detection biosensor with the chimeric protein system.</p> | ||
<p><a href="https://2010.igem.org/Image:Figure1.JPG" title="Image:Figure1.JPG"><img alt="Image:Figure1.JPG" src="https://static.igem.org/mediawiki/2010/1/1f/Figure1.JPG" width="610" height="233" border="0" /></a></p> | <p><a href="https://2010.igem.org/Image:Figure1.JPG" title="Image:Figure1.JPG"><img alt="Image:Figure1.JPG" src="https://static.igem.org/mediawiki/2010/1/1f/Figure1.JPG" width="610" height="233" border="0" /></a></p> | ||
<p><br /> | <p><br /> | ||
- | Signalling molecule CAI-1 binds to CqsS protein and this causes a change in the fusion receptor. This change triggers autophosphorylation of the histadine kinase BarA and it starts the phosphorylation pathway which ends in the response regulator UvrY. Phosphorylated UvrY acts as a transcription factor activating the promoter which causes the expression of the reporter gene.</p> | + | Signalling molecule CAI-1 binds to CqsS protein and this causes a change in the fusion receptor. This change triggers autophosphorylation of the histadine kinase BarA and it starts the phosphorylation pathway which ends in the response regulator UvrY.</p> |
+ | <p> Phosphorylated UvrY acts as a transcription factor activating the promoter which causes the expression of the reporter gene.</p> | ||
<p><br /> | <p><br /> | ||
<strong>Model for signal transduction</strong></p> | <strong>Model for signal transduction</strong></p> | ||
Line 118: | Line 116: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td height="28"><a href="https://2010.igem.org/File:GUI.zip"></a> </td> | + | <td width="850" height="28"><a href="https://2010.igem.org/File:GUI.zip"></a> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><p><a href="https://2010.igem.org/Team:Sheffield/Chimeric_protein_system" target="_self">Chimeric protein system</a> | <a href="https://2010.igem.org/Team:Sheffield/cholera_protein_system" target="_self">Cholera protein system</a> | <a href="https://2010.igem.org/Team:Sheffield/Graphical_User_Interface" target="_self">Graphical user interface</a></p></td> | + | <td width="850"><p><a href="https://2010.igem.org/Team:Sheffield/Chimeric_protein_system" target="_self">Chimeric protein system</a> | <a href="https://2010.igem.org/Team:Sheffield/cholera_protein_system" target="_self">Cholera protein system</a> | <a href="https://2010.igem.org/Team:Sheffield/Graphical_User_Interface" target="_self">Graphical user interface</a></p></td> |
</tr> | </tr> | ||
</table> | </table> | ||
</body> | </body> | ||
</html> | </html> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
[[Image:Sheffield sponsors.png]] | [[Image:Sheffield sponsors.png]] | ||
---- | ---- |
Revision as of 22:27, 25 October 2010