Team:UNIPV-Pavia/Calendar/July/settimana1

(Difference between revisions)

Revision as of 10:05, 5 July 2010

JULY: WEEK 1

Inoculum from glycerol stock for I7-1, I7-2, I8-1, I8-2, I9-1, I9-2, I10-1 and I10-2 in 5ml LB+Amp. Cultures were gown ON at 37°C 220rpm.

Inoculum of PhaP-1 and PhaP-2 in 5ml LB+Kan and ON growth at 37°C 220rpm.

We received strains and plasmid from ???Yale University???

name	description	growth conditions
BT340	pCP20 plasmid	LB+Amp LC 30°C,LB+Amp HC 30°C
BW23473	E. coli pir+ strain	LB, 37°C
BW23474	E. coli pir116 strain	LB, 37°C
BW25141	E. coli pir+ strain	LB, 37°C
BW25142	E. coli pir116 strain	LB, 37°C
BW5328 /pAH123	helper plasmid	LB+Amp LC 30°C,LB+Amp HC 30°C
MC1061	E. coli strain for integration	LB, 37°C
MG165	E. coli wil type strain	LB, 37°C

Screening of new colonies of I7 and I8. New screening for I9, I10

This time all the clones are OK :)

week 1

week 2

@@ Line 15: / Line 15: @@
 ==June, 28th==
+Inoculum from glycerol stock for I7-1, I7-2, I8-1, I8-2, I9-1, I9-2, I10-1 and I10-2 in 5ml LB+Amp. Cultures were gown ON at 37°C 220rpm.
+Inoculum of PhaP-1 and PhaP-2 in 5ml LB+Kan and ON growth at 37°C 220rpm.
+We received strains and plasmid from ???Yale University???
+{| border='1' align='center'
+|| '''name''' || '''description''' || '''growth conditions'''
+|-
+|| BT340 || pCP20 plasmid || LB+Amp LC 30°C,LB+Amp HC 30°C
+|-
+||BW23473 || ''E. coli'' pir+ strain||LB, 37°C
+|-
+||BW23474 || ''E. coli'' pir116 strain||LB, 37°C
+|-
+||BW25141 || ''E. coli'' pir+ strain||LB, 37°C
+|-
+||BW25142 || ''E. coli'' pir116 strain||LB, 37°C
+|-
+||BW5328 /pAH123 || helper plasmid ||LB+Amp LC 30°C,LB+Amp HC 30°C
+|-
+||MC1061 || ''E. coli'' strain for integration||LB, 37°C
+|-
+||MG165 || ''E. coli'' wil type strain||LB, 37°C
+|}
 ==June, 29th==