"
Team:Michigan/Pili Expression
From 2010.igem.org
(Difference between revisions)
| Line 19: | Line 19: | ||
|[[Team:Michigan/Pili_Expression#6/30/2010|6/30/2010]] | |[[Team:Michigan/Pili_Expression#6/30/2010|6/30/2010]] | ||
|[[Team:Michigan/Pili_Expression#7/1/2010|7/1/2010]] | |[[Team:Michigan/Pili_Expression#7/1/2010|7/1/2010]] | ||
| + | | - | ||
| + | | - | ||
| + | |- | ||
| + | !Week 2 | ||
| + | | - | ||
| + | | - | ||
| + | | - | ||
| + | | - | ||
| + | | - | ||
| - | | - | ||
| - | | - | ||
Revision as of 22:07, 6 July 2010
| Sunday | Monday | Tuesday | Wednesday | Thursday | Friday | Saturday | |
| Week 1 | - | 6/28/2010 | 6/29/2010 | 6/30/2010 | 7/1/2010 | - | - |
| Week 2 | - | - | - | - | - | - | - |
6/28/2010
Made a 500 mL batch of LB broth
- When pouring in distilled water, pour a few mL at a time to avoid clumping.
- It takes 20g of powder to make 1 L of broth
Sterilized broth using the autoclave
- The temperature setting on the autoclave is off by a little bit
- Set dial 2 notches below 134°C.
6/29/2010
Started growing E. coli K12 cultures
- Poured 2 mL of LB broth into a Falcon tube
- Used strain of K12 from Dr. Lin's freezer
- Placed in incubator shaker for 24 hrs.
Added and inventoried supplies from Dr. Pinto's lab.
- Regular trash can be thrown out by going down one floor, then going outside to the trash bins.
- Chemical wastes must be cleaned by calling OSEH.
6/30/2010
Cryopreserved stock of K12
- 1 mL located in the iGEM box in the Lin lab.
- 1 mL located in the lab freezer.
7/1/2010
Cryopreserved DH5α according to protocol procedure on 6/30/2010
- Put 1 stock in -20°C fridge in 1239
- Put the other stock in the -80°C fridge in the Lin lab
